2g79: Difference between revisions

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New page: left|200px<br /> <applet load="2g79" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g79, resolution 1.690Å" /> '''Crystal Structure ...
 
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[[Image:2g79.gif|left|200px]]<br />
<applet load="2g79" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2g79, resolution 1.690&Aring;" />
'''Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinal at 1.69 Angstroms Resolution'''<br />


==Overview==
==Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinal at 1.69 Angstroms Resolution==
Rational redesign of the binding pocket of Cellular Retinoic Acid Binding, Protein II (CRABPII) has provided a mutant that can bind retinal as a, protonated Schiff base, mimicking the binding observed in rhodopsin. The, reengineering was accomplished through a series of choreographed, manipulations to ultimately orient the reactive species (the epsilon-amino, group of Lys132 and the carbonyl of retinal) in the proper geometry for, imine formation. The guiding principle was to achieve the appropriate, Burgi-Dunitz trajectory for the reaction to ensue. Through, crystallographic analysis of protein mutants incapable of forming the, requisite Schiff base, a highly ordered water molecule was identified as a, key culprit in orienting retinal in a nonconstructive manner. Removal of, the ordered water, along with placing reinforcing mutations to favor the, desired orientation of retinal, led to a triple mutant CRABPII protein, capable of nanomolar binding of retinal as a protonated Schiff base. The, high-resolution crystal structure of all-trans-retinal bound to the, CRABPII triple mutant (1.2 A resolution) unequivocally illustrates the, imine formed between retinal and the protein.
<StructureSection load='2g79' size='340' side='right'caption='[[2g79]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2g79]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G79 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G79 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g79 OCA], [https://pdbe.org/2g79 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g79 RCSB], [https://www.ebi.ac.uk/pdbsum/2g79 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g79 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RABP2_HUMAN RABP2_HUMAN] Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/2g79_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g79 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2G79 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NA, SO4 and RET as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2G79 OCA].
*[[CRABP I ( Cellular Retinoic Acid Binding Protein )|CRABP I ( Cellular Retinoic Acid Binding Protein )]]
 
*[[Cellular retinoic acid-binding protein 3D structures|Cellular retinoic acid-binding protein 3D structures]]
==Reference==
*[[Gustavo Elberto Epalza Sanchez/Sandbox 1|Gustavo Elberto Epalza Sanchez/Sandbox 1]]
Protein design: reengineering cellular retinoic acid binding protein II into a rhodopsin protein mimic., Vasileiou C, Vaezeslami S, Crist RM, Rabago-Smith M, Geiger JH, Borhan B, J Am Chem Soc. 2007 May 16;129(19):6140-8. Epub 2007 Apr 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17447762 17447762]
*[[Molecular Playground/CRABP I (Cellular Retinoic Acid Binding Protein)|Molecular Playground/CRABP I (Cellular Retinoic Acid Binding Protein)]]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Geiger, J.H.]]
[[Category: Geiger JH]]
[[Category: Vaezeslami, S.]]
[[Category: Vaezeslami S]]
[[Category: NA]]
[[Category: RET]]
[[Category: SO4]]
[[Category: beta barrel]]
[[Category: crabpii]]
[[Category: crystallography]]
[[Category: high resolution]]
[[Category: retinal]]
[[Category: retinoic acid]]
[[Category: retinoids]]
[[Category: x-ray]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:15:54 2007''

Latest revision as of 12:26, 14 February 2024

Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinal at 1.69 Angstroms ResolutionCrystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinal at 1.69 Angstroms Resolution

Structural highlights

2g79 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.69Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RABP2_HUMAN Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2g79, resolution 1.69Å

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