2fz4: Difference between revisions

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New page: left|200px<br /><applet load="2fz4" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fz4, resolution 2.40Å" /> '''Crystal Structure of...
 
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[[Image:2fz4.gif|left|200px]]<br /><applet load="2fz4" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2fz4, resolution 2.40&Aring;" />
'''Crystal Structure of the N-terminal half of Archaeoglobus Fulgidus XPB'''<br />


==Overview==
==Crystal Structure of the N-terminal half of Archaeoglobus Fulgidus XPB==
The human xeroderma pigmentosum group B (XPB) helicase is essential for, transcription, nucleotide excision repair, and TFIIH functional assembly., Here, we determined crystal structures of an Archaeoglobus fulgidus XPB, homolog (AfXPB) that characterize two RecA-like XPB helicase domains and, discover a DNA damage recognition domain (DRD), a unique RED motif, a, flexible thumb motif (ThM), and implied conformational changes within a, conserved functional core. RED motif mutations dramatically reduce, helicase activity, and the DRD and ThM, which flank the RED motif, appear, structurally as well as functionally analogous to the MutS mismatch, recognition and DNA polymerase thumb domains. Substrate specificity is, altered by DNA damage, such that AfXPB unwinds dsDNA with 3' extensions, but not blunt-ended dsDNA, unless it contains a lesion, as shown for CPD, or (6-4) photoproducts. Together, these results provide an unexpected, mechanism of DNA unwinding with implications for XPB damage verification, in nucleotide excision repair.
<StructureSection load='2fz4' size='340' side='right'caption='[[2fz4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2fz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FZ4 FirstGlance]. <br>
2FZ4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZ4 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fz4 OCA], [https://pdbe.org/2fz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fz4 RCSB], [https://www.ebi.ac.uk/pdbsum/2fz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fz4 ProSAT]</span></td></tr>
==Reference==
</table>
Conserved XPB core structure and motifs for DNA unwinding: implications for pathway selection of transcription or excision repair., Fan L, Arvai AS, Cooper PK, Iwai S, Hanaoka F, Tainer JA, Mol Cell. 2006 Apr 7;22(1):27-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16600867 16600867]
== Function ==
[[Category: Archaeoglobus fulgidus dsm 4304]]
[https://www.uniprot.org/uniprot/O29889_ARCFU O29889_ARCFU]  
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Fan, L.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Tainer, J.A.]]
Check<jmol>
[[Category: dna damage recognition domain]]
  <jmolCheckbox>
[[Category: reca-like domain]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fz/2fz4_consurf.spt"</scriptWhenChecked>
 
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:47:58 2008''
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fz4 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Archaeoglobus fulgidus DSM 4304]]
[[Category: Large Structures]]
[[Category: Fan L]]
[[Category: Tainer JA]]

Latest revision as of 12:25, 14 February 2024

Crystal Structure of the N-terminal half of Archaeoglobus Fulgidus XPBCrystal Structure of the N-terminal half of Archaeoglobus Fulgidus XPB

Structural highlights

2fz4 is a 1 chain structure with sequence from Archaeoglobus fulgidus DSM 4304. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O29889_ARCFU

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

2fz4, resolution 2.40Å

Drag the structure with the mouse to rotate

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OCA
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