2fom: Difference between revisions

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-[[Image:2fom.gif|left|200px]]
- {{Structure
+==Dengue Virus NS2B/NS3 Protease==
-|PDB= 2fom |SIZE=350|CAPTION= <scene name='initialview01'>2fom</scene>, resolution 1.50&Aring;
+<StructureSection load='2fom' size='340' side='right'caption='[[2fom]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+<table><tr><td colspan='2'>[[2fom]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FOM FirstGlance]. <br>
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Flavivirin Flavivirin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.91 3.4.21.91] </span>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fom OCA], [https://pdbe.org/2fom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fom RCSB], [https://www.ebi.ac.uk/pdbsum/2fom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fom ProSAT]</span></td></tr>
-|RELATEDENTRY=
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fom OCA], [http://www.ebi.ac.uk/pdbsum/2fom PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fom RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/Q91H74_9FLAV Q91H74_9FLAV] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS026470_004_099774]
+== Evolutionary Conservation ==
-'''Dengue Virus NS2B/NS3 Protease'''
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
-==Overview==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/2fom_consurf.spt"</scriptWhenChecked>
-The replication of flaviviruses requires the correct processing of their polyprotein by the viral NS3 protease (NS3pro). Essential for the activation of NS3pro is a 47-residue region of NS2B. Here we report the crystal structures of a dengue NS2B-NS3pro complex and a West Nile virus NS2B-NS3pro complex with a substrate-based inhibitor. These structures identify key residues for NS3pro substrate recognition and clarify the mechanism of NS3pro activation.
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==About this Structure==
+  </jmolCheckbox>
-FOM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FOM OCA].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fom ConSurf].
+<div style="clear:both"></div>
-==Reference==
+__TOC__
-Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus., Erbel P, Schiering N, D'Arcy A, Renatus M, Kroemer M, Lim SP, Yin Z, Keller TH, Vasudevan SG, Hommel U, Nat Struct Mol Biol. 2006 Apr;13(4):372-3. Epub 2006 Mar 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16532006 16532006]
+</StructureSection>
 [[Category: Dengue virus 2]]
-[[Category: Flavivirin]]
+[[Category: Large Structures]]
-[[Category: Protein complex]]
+[[Category: D'Arcy A]]
-[[Category: Arcy, A D.]]
+[[Category: Erbel P]]
-[[Category: Erbel, P.]]
+[[Category: Kroemer M]]
-[[Category: Kroemer, M.]]
+[[Category: Renatus M]]
-[[Category: Renatus, M.]]
+[[Category: Schiering N]]
-[[Category: Schiering, N.]]
-[[Category: flavivirus]]
-[[Category: ns2b cofactor]]
-[[Category: ns3 protease]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:05:40 2008''