2fl8: Difference between revisions

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[[Image:2fl8.gif|left|200px]]


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==Fitting of the gp10 trimer structure into the cryoEM map of the bacteriophage T4 baseplate in the hexagonal conformation.==
The line below this paragraph, containing "STRUCTURE_2fl8", creates the "Structure Box" on the page.
<SX load='2fl8' size='340' side='right' viewer='molstar' caption='[[2fl8]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2fl8]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FL8 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fl8 OCA], [https://pdbe.org/2fl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fl8 RCSB], [https://www.ebi.ac.uk/pdbsum/2fl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fl8 ProSAT]</span></td></tr>
{{STRUCTURE_2fl8|  PDB=2fl8  |  SCENE= }}
</table>
 
== Function ==
'''Fitting of the gp10 trimer structure into the cryoEM map of the bacteriophage T4 baseplate in the hexagonal conformation.'''
[https://www.uniprot.org/uniprot/BP10_BPT4 BP10_BPT4] Baseplate protein that is part of the baseplate wedge and that connects the short tail fibers to the baseplate (PubMed:16554069). During infection, the baseplate undergoes a conformational change from a dome-shaped to a star-shaped structure. At this point, gp10 rotates and acts as a lever that unfolds the short tail fibers, which then interact with host cell surface receptors. Involved in the tail assembly.<ref>PMID:16554069</ref> <ref>PMID:21129200</ref>
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
The success of tailed bacteriophages to infect cells far exceeds that of most other viruses on account of their specialized tail and associated baseplate structures. The baseplate protein gene product (gp) 10 of bacteriophage T4, whose structure was determined to 1.2 A resolution, was fitted into the cryo-electron microscopy structures of the pre and post-infection conformations of the virus. gp10 functions as a molecular lever that rotates and extends the hinged short tail fibers to facilitate cell attachment. The central folding motif of the gp10 trimer is similar to that of the baseplate protein gp11 and to the receptor-binding domain of the short tail fiber, gp12. The three proteins comprise the periphery of the baseplate and interact with each other. The structural and functional similarities of gp10, gp11, and gp12 and their sequential order in the T4 genome suggest that they evolved separately, subsequent to gene triplication from a common ancestor. Such events are usual in the evolution of complex organelles from a common primordial molecule.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2fl8_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
2FL8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL8 OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fl8 ConSurf].
Evolution of bacteriophage tails: Structure of T4 gene product 10., Leiman PG, Shneider MM, Mesyanzhinov VV, Rossmann MG, J Mol Biol. 2006 May 5;358(3):912-21. Epub 2006 Mar 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16554069 16554069]
<div style="clear:both"></div>
[[Category: Enterobacteria phage t4]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Leiman, P G.]]
__TOC__
[[Category: Mesyanzhinov, V V.]]
</SX>
[[Category: Rossmann, M G.]]
[[Category: Escherichia virus T4]]
[[Category: Shneider, M M.]]
[[Category: Large Structures]]
[[Category: Bacteriophage t4]]
[[Category: Leiman PG]]
[[Category: Baseplate]]
[[Category: Mesyanzhinov VV]]
[[Category: Evolution]]
[[Category: Rossmann MG]]
[[Category: Gp10]]
[[Category: Shneider MM]]
[[Category: Structural comparison]]
[[Category: Tail]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 04:01:32 2008''

Latest revision as of 12:24, 14 February 2024

Fitting of the gp10 trimer structure into the cryoEM map of the bacteriophage T4 baseplate in the hexagonal conformation.Fitting of the gp10 trimer structure into the cryoEM map of the bacteriophage T4 baseplate in the hexagonal conformation.

2fl8, resolution 12.00Å

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