2f6h: Difference between revisions

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[[Image:2f6h.gif|left|200px]]


{{Structure
==Myosin V cargo binding domain==
|PDB= 2f6h |SIZE=350|CAPTION= <scene name='initialview01'>2f6h</scene>, resolution 2.25&Aring;
<StructureSection load='2f6h' size='340' side='right'caption='[[2f6h]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2f6h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F6H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F6H FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
|GENE= MYO2, CDC66 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f6h OCA], [https://pdbe.org/2f6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f6h RCSB], [https://www.ebi.ac.uk/pdbsum/2f6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f6h ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f6h OCA], [http://www.ebi.ac.uk/pdbsum/2f6h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f6h RCSB]</span>
[https://www.uniprot.org/uniprot/MYO2_YEAST MYO2_YEAST] Myosin heavy chain that is required for the cell cycle-regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Essential for the delivery of secretory vesicles to sites of active growth during bud emergence and cytokinesis. Required for segregation and inheritance of peroxisomes, late Golgi compartments, mitochondria and the vacuole to the daughter cell during cell division. Also required for correct alignment of the spindle during mitosis.<ref>PMID:10931864</ref> <ref>PMID:11285273</ref> <ref>PMID:11381095</ref> <ref>PMID:11733545</ref> <ref>PMID:11781333</ref> <ref>PMID:12391144</ref> <ref>PMID:12743102</ref> <ref>PMID:21248204</ref>
}}
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/2f6h_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f6h ConSurf].
<div style="clear:both"></div>


'''Myosin V cargo binding domain'''
==See Also==
 
*[[Myosin 3D Structures|Myosin 3D Structures]]
 
== References ==
==Overview==
<references/>
Myosin V molecular motors move cargoes on actin filaments. A myosin V may move multiple cargoes to distinct places at different times. The cargoes attach to the globular tail of myosin V via cargo-specific receptors. Here we report the crystal structure at 2.2 A of the myosin V globular tail. The overall tertiary structure has not been previously observed. There are several patches of highly conserved regions distributed on the surface of the tail. These are candidate attachment sites for cargo-specific receptors. Indeed, we identified a region of five conserved surface residues that are solely required for vacuole inheritance. Likewise, we identified a region of five conserved surface residues that are required for secretory vesicle movement, but not vacuole movement. These two regions are at opposite ends of the oblong-shaped cargo-binding domain, and moreover are offset by 180 degrees. The fact that the cargo-binding areas are distant from each other and simultaneously exposed on the surface of the globular tail suggests that major targets for the regulation of cargo attachment are organelle-specific myosin V receptors.
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2F6H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F6H OCA].
 
==Reference==
Structural basis for myosin V discrimination between distinct cargoes., Pashkova N, Jin Y, Ramaswamy S, Weisman LS, EMBO J. 2006 Feb 22;25(4):693-700. Epub 2006 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16437158 16437158]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Jin Y]]
[[Category: Jin, Y.]]
[[Category: Pashkova N]]
[[Category: Pashkova, N.]]
[[Category: Ramaswamy S]]
[[Category: Ramaswamy, S.]]
[[Category: Weisman LS]]
[[Category: Weisman, L S.]]
[[Category: cargo binding]]
[[Category: cargo transport]]
[[Category: mysoin v]]
[[Category: secreatory vescile binding]]
[[Category: vacuole binding]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:58:36 2008''

Latest revision as of 12:21, 14 February 2024

Myosin V cargo binding domainMyosin V cargo binding domain

Structural highlights

2f6h is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.25Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MYO2_YEAST Myosin heavy chain that is required for the cell cycle-regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Essential for the delivery of secretory vesicles to sites of active growth during bud emergence and cytokinesis. Required for segregation and inheritance of peroxisomes, late Golgi compartments, mitochondria and the vacuole to the daughter cell during cell division. Also required for correct alignment of the spindle during mitosis.[1] [2] [3] [4] [5] [6] [7] [8]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Catlett NL, Duex JE, Tang F, Weisman LS. Two distinct regions in a yeast myosin-V tail domain are required for the movement of different cargoes. J Cell Biol. 2000 Aug 7;150(3):513-26. PMID:10931864
  2. ↑ Rossanese OW, Reinke CA, Bevis BJ, Hammond AT, Sears IB, O'Connor J, Glick BS. A role for actin, Cdc1p, and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae. J Cell Biol. 2001 Apr 2;153(1):47-62. PMID:11285273
  3. ↑ Reck-Peterson SL, Tyska MJ, Novick PJ, Mooseker MS. The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors. J Cell Biol. 2001 May 28;153(5):1121-6. PMID:11381095
  4. ↑ Hoepfner D, van den Berg M, Philippsen P, Tabak HF, Hettema EH. A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae. J Cell Biol. 2001 Dec 10;155(6):979-90. Epub 2001 Dec 3. PMID:11733545 doi:http://dx.doi.org/10.1083/jcb.200107028
  5. ↑ Schott DH, Collins RN, Bretscher A. Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length. J Cell Biol. 2002 Jan 7;156(1):35-9. Epub 2002 Jan 7. PMID:11781333 doi:http://dx.doi.org/10.1083/jcb.200110086
  6. ↑ Itoh T, Watabe A, Toh-E A, Matsui Y. Complex formation with Ypt11p, a rab-type small GTPase, is essential to facilitate the function of Myo2p, a class V myosin, in mitochondrial distribution in Saccharomyces cerevisiae. Mol Cell Biol. 2002 Nov;22(22):7744-57. PMID:12391144
  7. ↑ Hwang E, Kusch J, Barral Y, Huffaker TC. Spindle orientation in Saccharomyces cerevisiae depends on the transport of microtubule ends along polarized actin cables. J Cell Biol. 2003 May 12;161(3):483-8. PMID:12743102 doi:http://dx.doi.org/10.1083/jcb.200302030
  8. ↑ Rossi G, Brennwald P. Yeast homologues of lethal giant larvae and type V myosin cooperate in the regulation of Rab-dependent vesicle clustering and polarized exocytosis. Mol Biol Cell. 2011 Mar 15;22(6):842-57. doi: 10.1091/mbc.E10-07-0570. Epub 2011 , Jan 19. PMID:21248204 doi:http://dx.doi.org/10.1091/mbc.E10-07-0570

2f6h, resolution 2.25Å

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