2ey4: Difference between revisions

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-[[Image:2ey4.gif|left|200px]]<br /><applet load="2ey4" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2ey4, resolution 2.11&Aring;" />
-'''Crystal Structure of a Cbf5-Nop10-Gar1 Complex'''<br />
-==Overview==
+==Crystal Structure of a Cbf5-Nop10-Gar1 Complex==
-H/ACA RNA-protein complexes, comprised of four proteins and an H/ACA guide RNA, modify ribosomal and small nuclear RNAs. The H/ACA proteins are also essential components of telomerase in mammals. Cbf5 is the H/ACA protein that catalyzes isomerization of uridine to pseudouridine in target RNAs. Mutations in human Cbf5 (dyskerin) lead to dyskeratosis congenita. Here, we describe the 2.1 A crystal structure of a specific complex of three archaeal H/ACA proteins, Cbf5, Nop10, and Gar1. Cbf5 displays structural properties that are unique among known pseudouridine synthases and are consistent with its distinct function in RNA-guided pseudouridylation. We also describe the previously unknown structures of both Nop10 and Gar1 and the structural basis for their essential roles in pseudouridylation. By using information from related structures, we have modeled the entire ribonucleoprotein complex including both guide and substrate RNAs. We have also identified a dyskeratosis congenita mutation cluster site within a modeled dyskerin structure.
+<StructureSection load='2ey4' size='340' side='right'caption='[[2ey4]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2ey4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] and [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EY4 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ey4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ey4 OCA], [https://pdbe.org/2ey4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ey4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ey4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ey4 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8U029_PYRFU Q8U029_PYRFU]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ey/2ey4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ey4 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-EY4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] and [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EY4 OCA].
+*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
+*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
-==Reference==
+*[[Ribosome biogenesis protein 3D structures|Ribosome biogenesis protein 3D structures]]
-Crystal structure of a Cbf5-Nop10-Gar1 complex and implications in RNA-guided pseudouridylation and dyskeratosis congenita., Rashid R, Liang B, Baker DL, Youssef OA, He Y, Phipps K, Terns RM, Terns MP, Li H, Mol Cell. 2006 Jan 20;21(2):249-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16427014 16427014]
+__TOC__
-[[Category: Protein complex]]
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Pyrococcus furiosus]]
-[[Category: Pyrococcus furiosus dsm 3638]]
+[[Category: Pyrococcus furiosus DSM 3638]]
-[[Category: Li, H.]]
+[[Category: Li H]]
-[[Category: Liang, B.]]
+[[Category: Liang B]]
-[[Category: Rashid, R.]]
+[[Category: Rashid R]]
-[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]]
-[[Category: ZN]]
-[[Category: protein structure initiative]]
-[[Category: psi]]
-[[Category: secsg]]
-[[Category: southeast collaboratory for structural genomics]]
-[[Category: structural genomics]]
-[[Category: trimeric complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:15:43 2008''