2ex5: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2ex5.gif|left|200px]]
-<!--
+==Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA==
-The line below this paragraph, containing "STRUCTURE_2ex5", creates the "Structure Box" on the page.
+<StructureSection load='2ex5' size='340' side='right'caption='[[2ex5]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2ex5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_moewusii Chlamydomonas moewusii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EX5 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-{{STRUCTURE_2ex5|  PDB=2ex5  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ex5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ex5 OCA], [https://pdbe.org/2ex5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ex5 RCSB], [https://www.ebi.ac.uk/pdbsum/2ex5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ex5 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DNE1_CHLMO DNE1_CHLMO] Endonuclease involved in intron homing. Recognizes a degenerate sequence of 17-19 bp to produce a staggered cut 5 bp downstream from the CeLSU.5 intron insertion site.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/2ex5_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ex5 ConSurf].
+<div style="clear:both"></div>
-'''Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA'''
+==See Also==
+*[[Endonuclease 3D structures|Endonuclease 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution.
+[[Category: Chlamydomonas moewusii]]
+[[Category: Large Structures]]
-==About this Structure==
+[[Category: Synthetic construct]]
-EX5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_eugametos Chlamydomonas eugametos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA].
+[[Category: Spiegel PC]]
+[[Category: Stoddard BL]]
-==Reference==
-The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold., Spiegel PC, Chevalier B, Sussman D, Turmel M, Lemieux C, Stoddard BL, Structure. 2006 May;14(5):869-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16698548 16698548]
-[[Category: Chlamydomonas eugametos]]
-[[Category: Single protein]]
-[[Category: Spiegel, P C.]]
-[[Category: Stoddard, B L.]]
-[[Category: Homing endonuclease]]
-[[Category: Homodimer]]
-[[Category: Laglidadg]]
-[[Category: Protein/dna complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 03:12:49 2008''