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| [[Image:2esg.gif|left|200px]]
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| {{Structure
| | ==Solution structure of the complex between immunoglobulin IgA1 and human serum albumin== |
| |PDB= 2esg |SIZE=350|CAPTION= <scene name='initialview01'>2esg</scene>
| | <StructureSection load='2esg' size='340' side='right'caption='[[2esg]]' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[2esg]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ESG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ESG FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray solution scattering</td></tr> |
| |GENE= ALB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2esg OCA], [https://pdbe.org/2esg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2esg RCSB], [https://www.ebi.ac.uk/pdbsum/2esg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2esg ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=[[1iga|1IGA]], [[1ao6|1AO6]]
| | == Disease == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2esg OCA], [http://www.ebi.ac.uk/pdbsum/2esg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2esg RCSB]</span>
| | [https://www.uniprot.org/uniprot/IGHA1_HUMAN IGHA1_HUMAN] Note=A chromosomal aberration involving IGHA1 is found in multiple myeloma (MM) cell lines. Translocation t(1;14)(q21;q32) that forms a FCRL4-IGHA1 fusion protein. |
| }}
| | == Function == |
| | [https://www.uniprot.org/uniprot/IGHA1_HUMAN IGHA1_HUMAN] Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. |
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/es/2esg_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2esg ConSurf]. |
| | <div style="clear:both"></div> |
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| '''Solution structure of the complex between immunoglobulin IgA1 and human serum albumin'''
| | ==See Also== |
| | | *[[Albumin 3D structures|Albumin 3D structures]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| Immunoglobulin A (IgA) is unique amongst antibodies in being able to form polymeric structures that may possess important functions in the pathology of specific diseases. IgA also forms complexes with other plasma proteins, the IgA1-human serum albumin (HSA) complex (IgA1-HSA) being typical. We have purified this complex using a novel two-step purification based on thiophilic chromatography and gel filtration, and characterised this. HSA is linked covalently to the tailpiece of IgA1 by a disulphide bond between Cys471 in IgA1 and Cys34 in HSA. IgA1-HSA binds to IgA receptors on neutrophils and monocytes, and elicits a respiratory burst that is comparable in magnitude to that of monomeric IgA1. The solution arrangement of IgA1-HSA was identified by X-ray scattering and ultracentrifugation. The radius of gyration R(G) of 7.5(+/-0.3) nm showed that IgA1-HSA is more extended in solution than IgA1 (R(G) of 6.1-6.2 nm). Its distance distribution function P(r) showed two peaks that indicated a well-separated solution structure similar to that for IgA1, and a maximum dimension of 25 nm, which is greater than that of 21 nm for IgA1. Sedimentation equilibrium showed that the IgA1:HSA stoichiometry is 1:1. Sedimentation velocity resulted in a sedimentation coefficient of 6.4S and a frictional ratio of 1.87, which is greater than that of 1.56 for IgA1. The constrained modelling of the IgA1-HSA structure using known structures for IgA1 and HSA generated 2432 conformationally randomised models of which 52 gave good scattering fits. The HSA structure was located at the base of the Fc fragment in IgA1 in an extended arrangement. Such a structure accounts for the functional activity of IgA1-HSA, and supports our previous modelling analysis of the IgA1 solution structure. The IgA1-HSA complex may suggest the potential for creating a new class of targeted therapeutic reagents based on the coupling of IgA1 to carrier proteins.
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| ==Disease==
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| Known disease associated with this structure: Analbuminemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=103600 103600]], Dysalbuminemic hyperthyroxinemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=103600 103600]], Dysalbuminemic hyperzincemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=103600 103600]]
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| ==About this Structure==
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| 2ESG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ESG OCA].
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| ==Reference==
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| Purification, properties and extended solution structure of the complex formed between human immunoglobulin A1 and human serum albumin by scattering and ultracentrifugation., Almogren A, Furtado PB, Sun Z, Perkins SJ, Kerr MA, J Mol Biol. 2006 Feb 17;356(2):413-31. Epub 2005 Dec 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16376934 16376934]
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| [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Almogren, A.]] | | [[Category: Almogren A]] |
| [[Category: Furtado, P B.]] | | [[Category: Furtado PB]] |
| [[Category: Kerr, M A.]] | | [[Category: Kerr MA]] |
| [[Category: Perkins, S J.]] | | [[Category: Perkins SJ]] |
| [[Category: Sun, Z.]] | | [[Category: Sun Z]] |
| [[Category: antibody]]
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| [[Category: human serum albumin]]
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| [[Category: immunoglobulin]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:53:10 2008''
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