2b1f: Difference between revisions

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New page: left|200px<br /><applet load="2b1f" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b1f, resolution 1.50Å" /> '''Antiparallel four-st...
 
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[[Image:2b1f.gif|left|200px]]<br /><applet load="2b1f" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2b1f, resolution 1.50&Aring;" />
'''Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat'''<br />


==Overview==
==Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat==
Coiled-coil sequences in proteins commonly share a seven-amino acid repeat, with nonpolar side chains at the first (a) and fourth (d) positions. We, investigate here the role of a 3-3-1 hydrophobic repeat containing, nonpolar amino acids at the a, d, and g positions in determining the, structures of coiled coils using mutants of the GCN4 leucine zipper, dimerization domain. When three charged residues at the g positions in the, parental sequence are replaced by nonpolar alanine or valine side chains, stable four-helix structures result. The X-ray crystal structures of the, tetramers reveal antiparallel, four-stranded coiled coils in which the a, d, and g side chains interlock in a combination of knobs-into-knobs and, knobs-into-holes packing. Interfacial interactions in a coiled coil can, therefore be prescribed by hydrophobic-polar patterns beyond the canonical, 3-4 heptad repeat. The results suggest that the conserved, charged, residues at the g positions in the GCN4 leucine zipper can impart a, negative design element to disfavor thermodynamically more stable, antiparallel tetramers.
<StructureSection load='2b1f' size='340' side='right'caption='[[2b1f]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2b1f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B1F FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b1f OCA], [https://pdbe.org/2b1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b1f RCSB], [https://www.ebi.ac.uk/pdbsum/2b1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b1f ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.


==About this Structure==
==See Also==
2B1F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2B1F OCA].
*[[Gcn4 3D Structures|Gcn4 3D Structures]]
 
*[[Gnc4 3D Structures|Gnc4 3D Structures]]
==Reference==
__TOC__
Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat., Deng Y, Liu J, Zheng Q, Eliezer D, Kallenbach NR, Lu M, Structure. 2006 Feb;14(2):247-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16472744 16472744]
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Deng Y]]
[[Category: Deng, Y.]]
[[Category: Eliezer D]]
[[Category: Eliezer, D.]]
[[Category: Kallenbach NR]]
[[Category: Kallenbach, N.R.]]
[[Category: Liu J]]
[[Category: Liu, J.]]
[[Category: Lu M]]
[[Category: Lu, M.]]
[[Category: Zheng Q]]
[[Category: Zheng, Q.]]
[[Category: ala coils]]
[[Category: antiparallel tetramer]]
[[Category: coiled coils]]
[[Category: protein design]]
[[Category: protein structure]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:31:11 2007''

Latest revision as of 12:15, 14 February 2024

Antiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeatAntiparallel four-stranded coiled coil specified by a 3-3-1 hydrophobic heptad repeat

Structural highlights

2b1f is a 4 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GCN4_YEAST Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.

See Also

2b1f, resolution 1.50Å

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