2azp: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{Seed}}
[[Image:2azp.png|left|200px]]


<!--
==Crystal Structure of PA1268 Solved by Sulfur SAD==
The line below this paragraph, containing "STRUCTURE_2azp", creates the "Structure Box" on the page.
<StructureSection load='2azp' size='340' side='right'caption='[[2azp]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2azp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AZP FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.13&#8491;</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2azp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2azp OCA], [https://pdbe.org/2azp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2azp RCSB], [https://www.ebi.ac.uk/pdbsum/2azp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2azp ProSAT], [https://www.topsan.org/Proteins/MCSG/2azp TOPSAN]</span></td></tr>
{{STRUCTURE_2azp|  PDB=2azp  |  SCENE=  }}
</table>
 
== Function ==
===Crystal Structure of PA1268 Solved by Sulfur SAD===
[https://www.uniprot.org/uniprot/4HYPE_PSEAE 4HYPE_PSEAE] Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting trans-4-hydroxy-L-proline (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp), which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways. Cannot use L-proline, trans-3-hydroxy-L-proline (t3LHyp) and pyrrolidone-5-carboxylate (P5C) as substrate.<ref>PMID:17849014</ref> <ref>PMID:20665524</ref> <ref>PMID:24980702</ref>
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==About this Structure==
Check<jmol>
2AZP is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZP OCA].  
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/az/2azp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2azp ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Edwards, A.]]
[[Category: Edwards A]]
[[Category: Gorodichtchenskaia, E.]]
[[Category: Gorodichtchenskaia E]]
[[Category: Joachimiak, A.]]
[[Category: Joachimiak A]]
[[Category: Liu, Y.]]
[[Category: Liu Y]]
[[Category: MCSG, Midwest Center for Structural Genomics.]]
[[Category: Pai EF]]
[[Category: Pai, E F.]]
[[Category: Savchenko A]]
[[Category: Savchenko, A.]]
[[Category: Skarina T]]
[[Category: Skarina, T.]]
[[Category: Yang C]]
[[Category: Yang, C.]]
[[Category: Mcsg]]
[[Category: Midwest center for structural genomic]]
[[Category: Pa1268,apc5861,sulfur sad]]
[[Category: Protein structure initiative]]
[[Category: Psi]]
[[Category: Structural genomic]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Nov 16 18:35:17 2008''

Latest revision as of 12:14, 14 February 2024

Crystal Structure of PA1268 Solved by Sulfur SADCrystal Structure of PA1268 Solved by Sulfur SAD

Structural highlights

2azp is a 1 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.13Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

4HYPE_PSEAE Allows intracellular utilization of 4-hydroxyproline, one of the major constituents of host collagen, by converting trans-4-hydroxy-L-proline (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp), which can be further metabolized by intracellular 4-hydroxy-D-proline oxidases. Strong B-cell mitogen. Plays an important role in the regulation of intra- and extracellular amino acid pools, allowing the bacterium to profit from host precursors and enzymatic pathways. Cannot use L-proline, trans-3-hydroxy-L-proline (t3LHyp) and pyrrolidone-5-carboxylate (P5C) as substrate.[1] [2] [3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Goytia M, Chamond N, Cosson A, Coatnoan N, Hermant D, Berneman A, Minoprio P. Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens. PLoS One. 2007 Sep 12;2(9):e885. PMID:17849014 doi:http://dx.doi.org/10.1371/journal.pone.0000885
  2. ↑ Gavina JM, White CE, Finan TM, Britz-McKibbin P. Determination of 4-hydroxyproline-2-epimerase activity by capillary electrophoresis: A stereoselective platform for inhibitor screening of amino acid isomerases. Electrophoresis. 2010 Aug;31(16):2831-7. doi: 10.1002/elps.201000187. PMID:20665524 doi:http://dx.doi.org/10.1002/elps.201000187
  3. ↑ Zhao S, Sakai A, Zhang X, Vetting MW, Kumar R, Hillerich B, San Francisco B, Solbiati J, Steves A, Brown S, Akiva E, Barber A, Seidel RD, Babbitt PC, Almo SC, Gerlt JA, Jacobson MP. Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks. Elife. 2014 Jun 30;3. doi: 10.7554/eLife.03275. PMID:24980702 doi:http://dx.doi.org/10.7554/eLife.03275

2azp, resolution 2.13Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=2azp&oldid=4057855"