2ayn: Difference between revisions

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OCA

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-[[Image:2ayn.gif|left|200px]]<br />
-<applet load="2ayn" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2ayn, resolution 3.2&Aring;" />
-'''Structure of USP14, a proteasome-associated deubiquitinating enzyme'''<br />
-==Overview==
+==Structure of USP14, a proteasome-associated deubiquitinating enzyme==
-The ubiquitin-specific processing protease (UBP) family of, deubiquitinating enzymes plays an essential role in numerous cellular, processes. Mammalian USP14 (Ubp6 in yeast) is unique among known UBP, enzymes in that it is activated catalytically upon specific association, with the 26S proteasome. Here, we report the crystal structures of the, 45-kDa catalytic domain of USP14 in isolation and in a complex with, ubiquitin aldehyde, which reveal distinct structural features. In the, absence of ubiquitin binding, the catalytic cleft leading to the active, site of USP14 is blocked by two surface loops. Binding by ubiquitin, induces a significant conformational change that translocates the two, surface loops thereby allowing access of the ubiquitin C-terminus to the, active site. These structural observations, in conjunction with, biochemical characterization, identify important regulatory mechanisms for, USP14.
+<StructureSection load='2ayn' size='340' side='right'caption='[[2ayn]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2ayn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AYN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AYN FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ayn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ayn OCA], [https://pdbe.org/2ayn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ayn RCSB], [https://www.ebi.ac.uk/pdbsum/2ayn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ayn ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/UBP14_HUMAN UBP14_HUMAN] Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).<ref>PMID:18162577</ref> <ref>PMID:19135427</ref> <ref>PMID:19106094</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ay/2ayn_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ayn ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-AYN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Ubiquitin_thiolesterase Ubiquitin thiolesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.15 3.1.2.15] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AYN OCA].
+*[[Thioesterase 3D structures|Thioesterase 3D structures]]
+== References ==
-==Reference==
+<references/>
-Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14., Hu M, Li P, Song L, Jeffrey PD, Chenova TA, Wilkinson KD, Cohen RE, Shi Y, EMBO J. 2005 Nov 2;24(21):3747-56. Epub 2005 Oct 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16211010 16211010]
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Ubiquitin thiolesterase]]
+[[Category: Hu M]]
-[[Category: Hu, M.]]
+[[Category: Jeffrey PD]]
-[[Category: Jeffrey, P.D.]]
+[[Category: Li P]]
-[[Category: Li, P.]]
+[[Category: Shi Y]]
-[[Category: Shi, Y.]]
-[[Category: deubiquitinating enzymes]]
-[[Category: dub]]
-[[Category: enzyme mechanism]]
-[[Category: proteasome]]
-[[Category: usp14]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:56:13 2007''