2aak: Difference between revisions

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-[[Image:2aak.jpg|left|200px]]
-<!--
+==UBIQUITIN CONJUGATING ENZYME FROM ARABIDOPSIS THALIANA==
-The line below this paragraph, containing "STRUCTURE_2aak", creates the "Structure Box" on the page.
+<StructureSection load='2aak' size='340' side='right'caption='[[2aak]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2aak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1aak 1aak]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AAK FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aak OCA], [https://pdbe.org/2aak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aak RCSB], [https://www.ebi.ac.uk/pdbsum/2aak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aak ProSAT]</span></td></tr>
-{{STRUCTURE_2aak|  PDB=2aak  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/UBC1_ARATH UBC1_ARATH] Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.<ref>PMID:16339806</ref> <ref>PMID:8386169</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aa/2aak_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aak ConSurf].
+<div style="clear:both"></div>
-'''UBIQUITIN CONJUGATING ENZYME FROM ARABIDOPSIS THALIANA'''
+==See Also==
+*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
+== References ==
-==Overview==
+<references/>
-The x-ray crystal structure of a recombinant ubiquitin-conjugating enzyme (E2) encoded by the UBC1 gene of the plant Arabidopsis thaliana has been determined with the use of multiple isomorphous replacement techniques and refined at 2.4-A resolution by simulated annealing and restrained least-squares. This E2 is an alpha/beta protein, with four alpha-helices and a four-stranded antiparallel beta-sheet. The NH2 and COOH termini, which may be important for interaction with other enzymes and substrates in the ubiquitin-conjugation pathway, are on the opposite side of the molecule from the cysteine residue that binds to the COOH terminus of ubiquitin. This structure should now allow for the rational analysis of E2 function by in vitro mutagenesis and facilitate the effective design of E2s with unique specificities or catalytic functions.
+__TOC__
+</StructureSection>
-==About this Structure==
-AAK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1aak 1aak]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AAK OCA].
-==Reference==
-Three-dimensional structure of a ubiquitin-conjugating enzyme (E2)., Cook WJ, Jeffrey LC, Sullivan ML, Vierstra RD, J Biol Chem. 1992 Jul 25;267(21):15116-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1321826 1321826]
 [[Category: Arabidopsis thaliana]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Ubiquitin--protein ligase]]
+[[Category: Cook WJ]]
-[[Category: Cook, W J.]]
+[[Category: Jeffrey LC]]
-[[Category: Jeffrey, L C.]]
+[[Category: Sullivan ML]]
-[[Category: Sullivan, M L.]]
+[[Category: Vierstra RD]]
-[[Category: Vierstra, R D.]]
-[[Category: Ligase]]
-[[Category: Ubiquitin conjugation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:49:06 2008''