2a7s: Difference between revisions

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-[[Image:2a7s.gif|left|200px]]<br /><applet load="2a7s" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2a7s, resolution 2.90&Aring;" />
-'''Crystal Structure of the Acyl-CoA Carboxylase, AccD5, from Mycobacterium tuberculosis'''<br />
-==Overview==
+==Crystal Structure of the Acyl-CoA Carboxylase, AccD5, from Mycobacterium tuberculosis==
-Mycolic acids and multimethyl-branched fatty acids are found uniquely in, the cell envelope of pathogenic mycobacteria. These unusually long fatty, acids are essential for the survival, virulence, and antibiotic resistance, of Mycobacterium tuberculosis. Acyl-CoA carboxylases (ACCases) commit, acyl-CoAs to the biosynthesis of these unique fatty acids. Unlike other, organisms such as Escherichia coli or humans that have only one or two, ACCases, M. tuberculosis contains six ACCase carboxyltransferase domains, AccD1-6, whose specific roles in the pathogen are not well defined., Previous studies indicate that AccD4, AccD5, and AccD6 are important for, cell envelope lipid biosynthesis and that its disruption leads to pathogen, death. We have determined the 2.9-Angstroms crystal structure of AccD5, whose sequence, structure, and active site are highly conserved with, respect to the carboxyltransferase domain of the Streptomyces coelicolor, propionyl-CoA carboxylase. Contrary to the previous proposal that AccD4-5, accept long-chain acyl-CoAs as their substrates, both crystal structure, and kinetic assay indicate that AccD5 prefers propionyl-CoA as its, substrate and produces methylmalonyl-CoA, the substrate for the, biosyntheses of multimethyl-branched fatty acids such as mycocerosic, phthioceranic, hydroxyphthioceranic, mycosanoic, and mycolipenic acids., Extensive in silico screening of National Cancer Institute compounds and, the University of California, Irvine, ChemDB database resulted in the, identification of one inhibitor with a K(i) of 13.1 microM. Our results, pave the way toward understanding the biological roles of key ACCases that, commit acyl-CoAs to the biosynthesis of cell envelope fatty acids, in, addition to providing a target for structure-based development of, antituberculosis therapeutics.
+<StructureSection load='2a7s' size='340' side='right'caption='[[2a7s]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[2a7s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A7S FirstGlance]. <br>
-A7S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/Propionyl-CoA_carboxylase Propionyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.3 6.4.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A7S OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7s OCA], [https://pdbe.org/2a7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a7s RCSB], [https://www.ebi.ac.uk/pdbsum/2a7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a7s ProSAT]</span></td></tr>
-==Reference==
+</table>
-Structure-based inhibitor design of AccD5, an essential acyl-CoA carboxylase carboxyltransferase domain of Mycobacterium tuberculosis., Lin TW, Melgar MM, Kurth D, Swamidass SJ, Purdon J, Tseng T, Gago G, Baldi P, Gramajo H, Tsai SC, Proc Natl Acad Sci U S A. 2006 Feb 28;103(9):3072-7. Epub 2006 Feb 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16492739 16492739]
+== Function ==
+[https://www.uniprot.org/uniprot/ACCD5_MYCTU ACCD5_MYCTU] Component of a biotin-dependent acyl-CoA carboxylase complex. This subunit transfers the CO2 from carboxybiotin to the CoA ester substrate (PubMed:16354663, PubMed:16385038, PubMed:28222482). When associated with the alpha3 subunit AccA3, is involved in the carboxylation of acetyl-CoA and propionyl-CoA, with a preference for propionyl-CoA (PubMed:16354663, PubMed:16385038, PubMed:28222482). Is also required for the activity of the long-chain acyl-CoA carboxylase (LCC) complex (PubMed:28222482).<ref>PMID:16354663</ref> <ref>PMID:16385038</ref> <ref>PMID:28222482</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/2a7s_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a7s ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Mycobacterium tuberculosis]]
-[[Category: Propionyl-CoA carboxylase]]
+[[Category: Lin T]]
-[[Category: Single protein]]
+[[Category: Melgar M]]
-[[Category: Lin, T.]]
+[[Category: Purdon J]]
-[[Category: Melgar, M.]]
+[[Category: Tsai SC]]
-[[Category: Purdon, J.]]
+[[Category: Tseng T]]
-[[Category: Tsai, S.C.]]
-[[Category: Tseng, T.]]
-[[Category: acetyl-coa carboxylase]]
-[[Category: acyl-coa carboxylase]]
-[[Category: carboxylase]]
-[[Category: carboxyltransferase]]
-[[Category: fatty acid]]
-[[Category: mycolic acid]]
-[[Category: polyketide]]
-[[Category: propionyl-coa carboxylase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:58:18 2007''