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[[Image:2a6m.gif|left|200px]]


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==Crystal Structure of the ISHp608 Transposase==
The line below this paragraph, containing "STRUCTURE_2a6m", creates the "Structure Box" on the page.
<StructureSection load='2a6m' size='340' side='right'caption='[[2a6m]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2a6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A6M FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a6m OCA], [https://pdbe.org/2a6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a6m RCSB], [https://www.ebi.ac.uk/pdbsum/2a6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a6m ProSAT]</span></td></tr>
{{STRUCTURE_2a6m|  PDB=2a6m |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q933Z0_HELPX Q933Z0_HELPX]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/2a6m_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a6m ConSurf].
<div style="clear:both"></div>


'''Crystal Structure of the ISHp608 Transposase'''
==See Also==
 
*[[Transposase 3D structures|Transposase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Many bacteria harbor simple transposable elements termed insertion sequences (IS). In Helicobacter pylori, the chimeric IS605 family elements are particularly interesting due to their proximity to genes encoding gastric epithelial invasion factors. Protein sequences of IS605 transposases do not bear the hallmarks of other well-characterized transposases. We have solved the crystal structure of full-length transposase (TnpA) of a representative member, ISHp608. Structurally, TnpA does not resemble any characterized transposase; rather, it is related to rolling circle replication (RCR) proteins. Consistent with RCR, Mg2+ and a conserved tyrosine, Tyr127, are essential for DNA nicking and the formation of a covalent intermediate between TnpA and DNA. TnpA is dimeric, contains two shared active sites, and binds two DNA stem loops representing the conserved inverted repeats near each end of ISHp608. The cocrystal structure with stem-loop DNA illustrates how this family of transposases specifically recognizes and pairs ends, necessary steps during transposition.
 
==About this Structure==
2A6M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6M OCA].
 
==Reference==
Active site sharing and subterminal hairpin recognition in a new class of DNA transposases., Ronning DR, Guynet C, Ton-Hoang B, Perez ZN, Ghirlando R, Chandler M, Dyda F, Mol Cell. 2005 Oct 7;20(1):143-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16209952 16209952]
[[Category: Helicobacter pylori]]
[[Category: Helicobacter pylori]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Chandler, M.]]
[[Category: Chandler M]]
[[Category: Dyda, F.]]
[[Category: Dyda F]]
[[Category: Ghirlando, R.]]
[[Category: Ghirlando R]]
[[Category: Guynet, C.]]
[[Category: Guynet C]]
[[Category: Perez, Z N.]]
[[Category: Perez ZN]]
[[Category: Ronning, D R.]]
[[Category: Ronning DR]]
[[Category: Ton-Hoang, B.]]
[[Category: Ton-Hoang B]]
[[Category: Rna recognition motif]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:40:21 2008''

Latest revision as of 12:11, 14 February 2024

Crystal Structure of the ISHp608 TransposaseCrystal Structure of the ISHp608 Transposase

Structural highlights

2a6m is a 2 chain structure with sequence from Helicobacter pylori. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q933Z0_HELPX

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2a6m, resolution 2.40Å

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