2a6m: Difference between revisions

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-[[Image:2a6m.gif|left|200px]]<br /><applet load="2a6m" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2a6m, resolution 2.40&Aring;" />
-'''Crystal Structure of the ISHp608 Transposase'''<br />
-==Overview==
+==Crystal Structure of the ISHp608 Transposase==
-Many bacteria harbor simple transposable elements termed insertion sequences (IS). In Helicobacter pylori, the chimeric IS605 family elements are particularly interesting due to their proximity to genes encoding gastric epithelial invasion factors. Protein sequences of IS605 transposases do not bear the hallmarks of other well-characterized transposases. We have solved the crystal structure of full-length transposase (TnpA) of a representative member, ISHp608. Structurally, TnpA does not resemble any characterized transposase; rather, it is related to rolling circle replication (RCR) proteins. Consistent with RCR, Mg2+ and a conserved tyrosine, Tyr127, are essential for DNA nicking and the formation of a covalent intermediate between TnpA and DNA. TnpA is dimeric, contains two shared active sites, and binds two DNA stem loops representing the conserved inverted repeats near each end of ISHp608. The cocrystal structure with stem-loop DNA illustrates how this family of transposases specifically recognizes and pairs ends, necessary steps during transposition.
+<StructureSection load='2a6m' size='340' side='right'caption='[[2a6m]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2a6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A6M FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a6m OCA], [https://pdbe.org/2a6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a6m RCSB], [https://www.ebi.ac.uk/pdbsum/2a6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a6m ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q933Z0_HELPX Q933Z0_HELPX]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/2a6m_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a6m ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-A6M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A6M OCA].
+*[[Transposase 3D structures|Transposase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Active site sharing and subterminal hairpin recognition in a new class of DNA transposases., Ronning DR, Guynet C, Ton-Hoang B, Perez ZN, Ghirlando R, Chandler M, Dyda F, Mol Cell. 2005 Oct 7;20(1):143-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16209952 16209952]
 [[Category: Helicobacter pylori]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Chandler, M.]]
+[[Category: Chandler M]]
-[[Category: Dyda, F.]]
+[[Category: Dyda F]]
-[[Category: Ghirlando, R.]]
+[[Category: Ghirlando R]]
-[[Category: Guynet, C.]]
+[[Category: Guynet C]]
-[[Category: Perez, Z N.]]
+[[Category: Perez ZN]]
-[[Category: Ronning, D R.]]
+[[Category: Ronning DR]]
-[[Category: Ton-Hoang, B.]]
+[[Category: Ton-Hoang B]]
-[[Category: rna recognition motif]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:24:07 2008''