2a58: Difference between revisions

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-[[Image:2a58.gif|left|200px]]<br /><applet load="2a58" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2a58, resolution 2.800&Aring;" />
-'''Structure of 6,7-Dimethyl-8-ribityllumazine synthase from Schizosaccharomyces pombe mutant W27Y with bound riboflavin'''<br />
-==Overview==
+==Structure of 6,7-Dimethyl-8-ribityllumazine synthase from Schizosaccharomyces pombe mutant W27Y with bound riboflavin==
-The amino acid residue tryptophan 27 of 6,7-dimethyl-8-ribityllumazine synthase of the yeast Schizosaccharomyces pombe was replaced by tyrosine. The structures of the W27Y mutant protein in complex with riboflavin, the substrate analogue 5-nitroso-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, and the product analogue 6-carboxyethyl-7-oxo-8-ribityllumazine, were determined by X-ray crystallography at resolutions of 2.7-2.8 A. Whereas the indole system of W27 forms a coplanar pi-complex with riboflavin, the corresponding phenyl ring in the W27Y mutant establishes only peripheral contact with the heterocyclic ring system of the bound riboflavin. These findings provide an explanation for the absence of the long wavelength shift in optical absorption spectra of riboflavin bound to the mutant enzyme. The structures of the mutants are important tools for the interpretation of the unusual physical properties of riboflavin in complex with lumazine synthase.
+<StructureSection load='2a58' size='340' side='right'caption='[[2a58]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[2a58]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A58 FirstGlance]. <br>
-A58 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=RBF:'>RBF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A58 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a58 OCA], [https://pdbe.org/2a58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a58 RCSB], [https://www.ebi.ac.uk/pdbsum/2a58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a58 ProSAT]</span></td></tr>
-Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase., Koch M, Breithaupt C, GerhardtHaase S, Weber S, Cushman M, Huber R, Bacher A, Fischer M, Eur J Biochem. 2004 Aug;271(15):3208-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15265040 15265040]
+</table>
-[[Category: Riboflavin synthase]]
+== Function ==
+[https://www.uniprot.org/uniprot/RIB4_SCHPO RIB4_SCHPO] Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. Also binds riboflavin with an unexpected high affinity.<ref>PMID:11856310</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/2a58_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a58 ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Schizosaccharomyces pombe]]
-[[Category: Single protein]]
+[[Category: Bacher A]]
-[[Category: Bacher, A.]]
+[[Category: Breithaupt C]]
-[[Category: Breithaupt, C.]]
+[[Category: Cushman M]]
-[[Category: Cushman, M.]]
+[[Category: Fischer M]]
-[[Category: Fischer, M.]]
+[[Category: Gerhardt S]]
-[[Category: Gerhardt, S.]]
+[[Category: Haase I]]
-[[Category: Haase, I.]]
+[[Category: Huber R]]
-[[Category: Huber, R.]]
+[[Category: Koch M]]
-[[Category: Koch, M.]]
+[[Category: Weber S]]
-[[Category: Weber, S.]]
-[[Category: PO4]]
-[[Category: RBF]]
-[[Category: 6]]
-[[Category: 7-dimethyl-8-ribityllumazine synthase]]
-[[Category: lusy]]
-[[Category: riboflavin]]
-[[Category: riboflavin biosynthesis]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:23:51 2008''