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[[Image:2a11.gif|left|200px]]


{{Structure
==Crystal Structure of Nuclease Domain of Ribonuclase III from Mycobacterium Tuberculosis==
|PDB= 2a11 |SIZE=350|CAPTION= <scene name='initialview01'>2a11</scene>, resolution 2.100&Aring;
<StructureSection load='2a11' size='340' side='right'caption='[[2a11]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
<table><tr><td colspan='2'>[[2a11]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A11 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A11 FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
|GENE= rnc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a11 OCA], [https://pdbe.org/2a11 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a11 RCSB], [https://www.ebi.ac.uk/pdbsum/2a11 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a11 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RNC_MYCTU RNC_MYCTU] Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism (By similarity).[HAMAP-Rule:MF_00104]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a1/2a11_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a11 ConSurf].
<div style="clear:both"></div>


'''Crystal Structure of Nuclease Domain of Ribonuclase III from Mycobacterium Tuberculosis'''
==See Also==
 
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
RNase III enzymes are a highly conserved family of proteins that specifically cleave double-stranded (ds)RNA. These proteins are involved in a diverse group of functions, including ribosomal RNA processing, mRNA maturation and decay, snRNA and snoRNA processing, and RNA interference. Here we report the crystal structure of the nuclease domain of RNase III from the pathogen Mycobacterium tuberculosis. Although globally similar to other RNase III folds, this structure has some features not observed in previously reported models. These include the presence of an additional metal ion near the catalytic site, as well as conserved secondary structural elements that are proposed to have functional roles in the recognition of dsRNAs.
[[Category: Large Structures]]
 
==About this Structure==
2A11 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A11 OCA].
 
==Reference==
Structure of the nuclease domain of ribonuclease III from M. tuberculosis at 2.1 A., Akey DL, Berger JM, Protein Sci. 2005 Oct;14(10):2744-50. Epub 2005 Sep 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16155207 16155207]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Ribonuclease III]]
[[Category: Akey DL]]
[[Category: Single protein]]
[[Category: Berger JM]]
[[Category: Akey, D L.]]
[[Category: Berger, J M.]]
[[Category: XMTB, Mycobacterium Tuberculosis Structural Proteomics Project.]]
[[Category: CA]]
[[Category: protein structure initiative; mycobacterium tuberculosis structural proteomics project (xmtb)]]
[[Category: psi]]
[[Category: ribonuclease; rnase iii; nuclease domain; structural genomic]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:44:07 2008''

Latest revision as of 12:10, 14 February 2024

Crystal Structure of Nuclease Domain of Ribonuclase III from Mycobacterium TuberculosisCrystal Structure of Nuclease Domain of Ribonuclase III from Mycobacterium Tuberculosis

Structural highlights

2a11 is a 1 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RNC_MYCTU Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism (By similarity).[HAMAP-Rule:MF_00104]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2a11, resolution 2.10Å

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