1zs4: Difference between revisions

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-[[Image:1zs4.gif|left|200px]]<br /><applet load="1zs4" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1zs4, resolution 1.7&Aring;" />
-'''Structure of bacteriophage lambda cII protein in complex with DNA'''<br />
-==Overview==
+==Structure of bacteriophage lambda cII protein in complex with DNA==
-The tetrameric cII protein from bacteriophage lambda activates transcription from the phage promoters P(RE), P(I), and P(AQ) by binding to two direct repeats that flank the promoter -35 element. Here, we present the X-ray crystal structure of cII alone (2.8 A resolution) and in complex with its DNA operator from P(RE) (1.7 A resolution). The structures provide a basis for modeling of the activation complex with the RNA polymerase holoenzyme, and point to the key role for the RNA polymerase alpha subunit C-terminal domain (alphaCTD) in cII-dependent activation, which forms a bridge of protein/protein interactions between cII and the RNA polymerase sigma subunit. The model makes specific predictions for protein/protein interactions between cII and alphaCTD, and between alphaCTD and sigma, which are supported by previous genetic studies.
+<StructureSection load='1zs4' size='340' side='right'caption='[[1zs4]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1zs4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZS4 FirstGlance]. <br>
-ZS4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZS4 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zs4 OCA], [https://pdbe.org/1zs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zs4 RCSB], [https://www.ebi.ac.uk/pdbsum/1zs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zs4 ProSAT]</span></td></tr>
-==Reference==
+</table>
-Crystal structure of bacteriophage lambda cII and its DNA complex., Jain D, Kim Y, Maxwell KL, Beasley S, Zhang R, Gussin GN, Edwards AM, Darst SA, Mol Cell. 2005 Jul 22;19(2):259-69. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16039594 16039594]
+== Function ==
-[[Category: Enterobacteria phage lambda]]
+[https://www.uniprot.org/uniprot/RPC2_LAMBD RPC2_LAMBD] This protein and protein CIII form a complex that is involved in the initiation of lysogeny. The complex binds at either of the cy regulatory sites preceding the CI repressor protein and integrase coding regions and induces transcription of these genes.
-[[Category: Single protein]]
+== Evolutionary Conservation ==
-[[Category: Beasley, S.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Darst, S A.]]
+Check<jmol>
-[[Category: Edwards, A M.]]
+  <jmolCheckbox>
-[[Category: Gussin, G N.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zs/1zs4_consurf.spt"</scriptWhenChecked>
-[[Category: Jain, D.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Kim, Y.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: Maxwell, K L.]]
+  </jmolCheckbox>
-[[Category: helix-turn-helix]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zs4 ConSurf].
-[[Category: transcription activator]]
+<div style="clear:both"></div>
+__TOC__
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:18:33 2008''
+</StructureSection>
+[[Category: Escherichia virus Lambda]]
+[[Category: Large Structures]]
+[[Category: Beasley S]]
+[[Category: Darst SA]]
+[[Category: Edwards AM]]
+[[Category: Gussin GN]]
+[[Category: Jain D]]
+[[Category: Kim Y]]
+[[Category: Maxwell KL]]