1zes: Difference between revisions

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New page: left|200px<br /><applet load="1zes" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zes, resolution 1.90Å" /> '''BeF3- activated PhoB...
 
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[[Image:1zes.gif|left|200px]]<br /><applet load="1zes" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zes, resolution 1.90&Aring;" />
'''BeF3- activated PhoB receiver domain'''<br />


==Overview==
==BeF3- activated PhoB receiver domain==
Response regulators (RRs), which undergo phosphorylation/dephosphorylation, at aspartate residues, are highly prevalent in bacterial signal, transduction. RRs typically contain an N-terminal receiver domain that, regulates the activities of a C-terminal DNA binding domain in a, phosphorylation-dependent manner. We present crystallography and solution, NMR data for the receiver domain of Escherichia coli PhoB which show, distinct 2-fold symmetric dimers in the inactive and active states. These, structures, together with the previously determined structure of the, C-terminal domain of PhoB bound to DNA, define the conformation of the, active transcription factor and provide a model for the mechanism of, activation in the OmpR/PhoB subfamily, the largest group of RRs. In the, active state, the receiver domains dimerize with 2-fold rotational, symmetry using their alpha4-beta5-alpha5 faces, while the effector domains, bind to DNA direct repeats with tandem symmetry, implying a loss of, intramolecular interactions.
<StructureSection load='1zes' size='340' side='right'caption='[[1zes]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1zes]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The October 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Two-component Systems''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_10 10.2210/rcsb_pdb/mom_2015_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZES FirstGlance]. <br>
1ZES is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and BEF as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZES OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zes OCA], [https://pdbe.org/1zes PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zes RCSB], [https://www.ebi.ac.uk/pdbsum/1zes PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zes ProSAT]</span></td></tr>
Mechanism of activation for transcription factor PhoB suggested by different modes of dimerization in the inactive and active states., Bachhawat P, Swapna GV, Montelione GT, Stock AM, Structure. 2005 Sep;13(9):1353-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16154092 16154092]
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHOB_ECOLI PHOB_ECOLI] This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ze/1zes_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zes ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bachhawat, P.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Montelione, G.T.]]
[[Category: Two-component Systems]]
[[Category: Stock, A.M.]]
[[Category: Bachhawat P]]
[[Category: BEF]]
[[Category: Montelione GT]]
[[Category: MG]]
[[Category: Stock AM]]
[[Category: activated]]
[[Category: chey-like fold]]
[[Category: phob]]
[[Category: response regulator]]
[[Category: transcription factor]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:25:07 2007''

Latest revision as of 12:04, 14 February 2024

BeF3- activated PhoB receiver domainBeF3- activated PhoB receiver domain

Structural highlights

1zes is a 3 chain structure with sequence from Escherichia coli. The October 2015 RCSB PDB Molecule of the Month feature on Two-component Systems by David Goodsell is 10.2210/rcsb_pdb/mom_2015_10. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PHOB_ECOLI This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1zes, resolution 1.90Å

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