1ynm: Difference between revisions

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New page: left|200px<br /><applet load="1ynm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ynm, resolution 2.65Å" /> '''Crystal structure of...
 
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[[Image:1ynm.gif|left|200px]]<br /><applet load="1ynm" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ynm, resolution 2.65&Aring;" />
'''Crystal structure of restriction endonuclease HinP1I'''<br />


==Overview==
==Crystal structure of restriction endonuclease HinP1I==
HinP1I, a type II restriction endonuclease, recognizes and cleaves a, palindromic tetranucleotide sequence (G/CGC) in double-stranded DNA, producing 2 nt 5' overhanging ends. Here, we report the structure of, HinP1I crystallized as one protein monomer in the crystallographic, asymmetric unit. HinP1I displays an elongated shape, with a conserved, catalytic core domain containing an active-site motif of SDX18QXK and a, putative DNA-binding domain. Without significant sequence homology, HinP1I, displays striking structural similarity to MspI, an endonuclease that, cleaves a similar palindromic DNA sequence (C/CGG) and binds to that, sequence crystallographically as a monomer. Almost all the structural, elements of MspI can be matched in HinP1I, including both the DNA, recognition and catalytic elements. Examining the protein-protein, interactions in the crystal lattice, HinP1I could be dimerized through two, helices located on the opposite side of the protein to the active site, generating a molecule with two active sites and two DNA-binding surfaces, opposite one another on the outer surfaces of the dimer. A possible, functional link between this unusual dimerization mode and the tetrameric, restriction enzymes is discussed.
<StructureSection load='1ynm' size='340' side='right'caption='[[1ynm]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ynm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ynm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ynm OCA], [https://pdbe.org/1ynm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ynm RCSB], [https://www.ebi.ac.uk/pdbsum/1ynm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ynm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5I6E6_HAEIF Q5I6E6_HAEIF]


==About this Structure==
==See Also==
1YNM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YNM OCA].
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of HinP1I endonuclease reveals a striking similarity to the monomeric restriction enzyme MspI., Yang Z, Horton JR, Maunus R, Wilson GG, Roberts RJ, Cheng X, Nucleic Acids Res. 2005 Apr 1;33(6):1892-901. Print 2005. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805123 15805123]
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Cheng X]]
[[Category: Cheng, X.]]
[[Category: Horton JR]]
[[Category: Horton, J.R.]]
[[Category: Maunus R]]
[[Category: Maunus, R.]]
[[Category: Roberts RJ]]
[[Category: Roberts, R.J.]]
[[Category: Wilson GG]]
[[Category: Wilson, G.G.]]
[[Category: Yang Z]]
[[Category: Yang, Z.]]
[[Category: dimerizaton]]
[[Category: restriction endonuclease]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:24:56 2007''

Latest revision as of 11:58, 14 February 2024

Crystal structure of restriction endonuclease HinP1ICrystal structure of restriction endonuclease HinP1I

Structural highlights

1ynm is a 1 chain structure with sequence from Haemophilus influenzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.65Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q5I6E6_HAEIF

See Also

1ynm, resolution 2.65Å

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