1xte: Difference between revisions

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OCA

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-[[Image:1xte.gif|left|200px]]<br /><applet load="1xte" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1xte, resolution 1.60&Aring;" />
-'''crystal structure of CISK-PX domain'''<br />
-==Overview==
+==crystal structure of CISK-PX domain==
-The cytokine-independent survival kinase (CISK) in the serum and, glucocorticoid-regulated kinase family plays an important role in, mediating cell growth and survival. N-terminal to its catalytic kinase, domain, CISK contains a phox homology (PX) domain, a, phosphoinositide-binding motif that directs the membrane localization of, CISK and regulates CISK activity. We have determined the crystal, structures of the mouse CISK-PX domain to unravel the structural basis of, membrane targeting of CISK. In addition to the specific interactions, conferred by the phosphoinositide-binding pocket, the structure suggests, that a hydrophobic loop region and a hydrophilic beta-turn contribute to, the interactions with the membrane. Furthermore, biochemical studies, reveal that CISK-PX dimerizes in the presence of the linker between the PX, domain and kinase domain, suggesting a multivalent mechanism in membrane, localization of CISK.
+<StructureSection load='1xte' size='340' side='right'caption='[[1xte]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1xte]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XTE FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xte OCA], [https://pdbe.org/1xte PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xte RCSB], [https://www.ebi.ac.uk/pdbsum/1xte PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xte ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SGK3_MOUSE SGK3_MOUSE] Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.<ref>PMID:15774536</ref> <ref>PMID:15774535</ref> <ref>PMID:21113728</ref> <ref>PMID:21451460</ref> <ref>PMID:21865597</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xt/1xte_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xte ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-XTE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XTE OCA].
+*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
+== References ==
-==Reference==
+<references/>
-Structural basis of membrane targeting by the Phox homology domain of cytokine-independent survival kinase (CISK-PX)., Xing Y, Liu D, Zhang R, Joachimiak A, Songyang Z, Xu W, J Biol Chem. 2004 Jul 16;279(29):30662-9. Epub 2004 May 4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15126499 15126499]
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Non-specific serine/threonine protein kinase]]
+[[Category: Joachimiak A]]
-[[Category: Single protein]]
+[[Category: Liu D]]
-[[Category: Joachimiak, A.]]
+[[Category: Songyang Z]]
-[[Category: Liu, D]]
+[[Category: Xing Y]]
-[[Category: Songyang, Z.]]
+[[Category: Xu W]]
-[[Category: Xing, Y.]]
+[[Category: Zhang R]]
-[[Category: Xu, W.]]
-[[Category: Zhang, R.]]
-[[Category: cisk]]
-[[Category: crystal structure]]
-[[Category: px domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:19:57 2007''