1xks: Difference between revisions

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New page: left|200px<br /> <applet load="1xks" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xks, resolution 2.35Å" /> '''The crystal structu...
 
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[[Image:1xks.gif|left|200px]]<br />
<applet load="1xks" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xks, resolution 2.35&Aring;" />
'''The crystal structure of the N-terminal domain of Nup133 reveals a beta-propeller fold common to several nucleoporins'''<br />


==Overview==
==The crystal structure of the N-terminal domain of Nup133 reveals a beta-propeller fold common to several nucleoporins==
Nucleocytoplasmic transport occurs through nuclear pore complexes (NPCs), whose complex architecture is generated from a set of only approximately, 30 proteins, termed nucleoporins. Here, we explore the domain structure of, Nup133, a nucleoporin in a conserved NPC subcomplex that is crucial for, NPC biogenesis and is believed to form part of the NPC scaffold. We show, that human Nup133 contains two domains: a COOH-terminal domain responsible, for its interaction with its subcomplex through Nup107; and an, NH2-terminal domain whose crystal structure reveals a seven-bladed, beta-propeller. The surface properties and conservation of the Nup133, beta-propeller suggest it may mediate multiple interactions with other, proteins. Other beta-propellers are predicted in a third of all, nucleoporins. These and several other repeat-based motifs appear to be, major elements of nucleoporins, indicating a level of structural, repetition that may conceptually simplify the assembly and disassembly of, this huge protein complex.
<StructureSection load='1xks' size='340' side='right'caption='[[1xks]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1xks]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XKS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xks OCA], [https://pdbe.org/1xks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xks RCSB], [https://www.ebi.ac.uk/pdbsum/1xks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xks ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NU133_HUMAN NU133_HUMAN] Involved in poly(A)+ RNA transport.<ref>PMID:11684705</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xk/1xks_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xks ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1XKS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XKS OCA].
*[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
 
== References ==
==Reference==
<references/>
Structural and functional analysis of Nup133 domains reveals modular building blocks of the nuclear pore complex., Berke IC, Boehmer T, Blobel G, Schwartz TU, J Cell Biol. 2004 Nov 22;167(4):591-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15557116 15557116]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Berke, I.C.]]
[[Category: Berke IC]]
[[Category: Blobel, G.]]
[[Category: Blobel G]]
[[Category: Boehmer, T.]]
[[Category: Boehmer T]]
[[Category: Schwartz, T.U.]]
[[Category: Schwartz TU]]
[[Category: beta-propeller]]
[[Category: helical insertions]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:07:05 2007''

Latest revision as of 11:51, 14 February 2024

The crystal structure of the N-terminal domain of Nup133 reveals a beta-propeller fold common to several nucleoporinsThe crystal structure of the N-terminal domain of Nup133 reveals a beta-propeller fold common to several nucleoporins

Structural highlights

1xks is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.35Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NU133_HUMAN Involved in poly(A)+ RNA transport.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Vasu S, Shah S, Orjalo A, Park M, Fischer WH, Forbes DJ. Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export. J Cell Biol. 2001 Oct 29;155(3):339-54. Epub 2001 Oct 29. PMID:11684705 doi:http://dx.doi.org/10.1083/jcb.200108007

1xks, resolution 2.35Å

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