1xa6: Difference between revisions

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-[[Image:1xa6.gif|left|200px]]<br /><applet load="1xa6" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1xa6, resolution 3.2&Aring;" />
-'''Crystal Structure of the Human Beta2-Chimaerin'''<br />
-==Overview==
+==Crystal Structure of the Human Beta2-Chimaerin==
-The lipid second messenger diacylglycerol acts by binding to the C1 domains of target proteins, which translocate to cell membranes and are allosterically activated. Here we report the crystal structure at 3.2 A resolution of one such protein, beta2-chimaerin, a GTPase-activating protein for the small GTPase Rac, in its inactive conformation. The structure shows that in the inactive state, the N terminus of beta2-chimaerin protrudes into the active site of the RacGAP domain, sterically blocking Rac binding. The diacylglycerol and phospholipid membrane binding site on the C1 domain is buried by contacts with the four different regions of beta2-chimaerin: the N terminus, SH2 domain, RacGAP domain, and the linker between the SH2 and C1 domains. Phospholipid binding to the C1 domain triggers the cooperative dissociation of these interactions, allowing the N terminus to move out of the active site and thereby activating the enzyme.
+<StructureSection load='1xa6' size='340' side='right'caption='[[1xa6]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+== Structural highlights ==
-==Disease==
+<table><tr><td colspan='2'>[[1xa6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XA6 FirstGlance]. <br>
-Known disease associated with this structure: Apnea, postanesthetic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=177400 177400]]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-==About this Structure==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xa6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xa6 OCA], [https://pdbe.org/1xa6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xa6 RCSB], [https://www.ebi.ac.uk/pdbsum/1xa6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xa6 ProSAT]</span></td></tr>
-XA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA6 OCA].
+</table>
+== Function ==
-==Reference==
+[https://www.uniprot.org/uniprot/CHIO_HUMAN CHIO_HUMAN] GTPase-activating protein for p21-rac. Insufficient expression of beta-2 chimaerin is expected to lead to higher Rac activity and could therefore play a role in the progression from low-grade to high-grade tumors.
-Structural mechanism for lipid activation of the Rac-specific GAP, beta2-chimaerin., Canagarajah B, Leskow FC, Ho JY, Mischak H, Saidi LF, Kazanietz MG, Hurley JH, Cell. 2004 Oct 29;119(3):407-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15507211 15507211]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xa/1xa6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xa6 ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Canagarajah, B.]]
+[[Category: Canagarajah B]]
-[[Category: Ho, J Y.]]
+[[Category: Ho JY]]
-[[Category: Hurley, J H.]]
+[[Category: Hurley JH]]
-[[Category: Kazanietz, M G.]]
+[[Category: Kazanietz MG]]
-[[Category: Leskow, F C.]]
+[[Category: Leskow FC]]
-[[Category: Mischak, H.]]
+[[Category: Mischak H]]
-[[Category: Saidi, L F.]]
+[[Category: Saidi LF]]
-[[Category: ZN]]
-[[Category: beta2-chimaerin]]
-[[Category: c1]]
-[[Category: racgap]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:39 2008''