1xa2: Difference between revisions

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<StructureSection load='1xa2' size='340' side='right'caption='[[1xa2]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
<StructureSection load='1xa2' size='340' side='right'caption='[[1xa2]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1xa2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XA2 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1xa2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XA2 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xa2 OCA], [http://pdbe.org/1xa2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xa2 RCSB], [http://www.ebi.ac.uk/pdbsum/1xa2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xa2 ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xa2 OCA], [https://pdbe.org/1xa2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xa2 RCSB], [https://www.ebi.ac.uk/pdbsum/1xa2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xa2 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report here the crystal structure of the DNA hexamer duplex d(CGCGCA)*d(TGCGCG) at 1.71 A resolution. The crystals, in orthorhombic space group, were grown in the presence of cobalt hexammine, a known inducer of the left-handed Z form of DNA. The interaction of this ion with the DNA helix results in a change of the adenine base from the common amino tautomeric form to the imino tautomer. Consequently the A:T base pair is disrupted from the normal Watson-Crick base pairing to a 'wobble' like base pairing. This change is accommodated easily within the helix, and the helical parameters are those expected for Z-DNA. When the cobalt hexammine concentration is decreased slightly in the crystallization conditions, the duplex crystallizes in a different, hexagonal space group, with two hexamer duplexes in the asymmetric unit. One of these is situated on a crystallographic 6-fold screw axis, leading to disorder. The tautomeric shift is not observed in this space group. We show that the change in inter-helix interactions that lead to the two different space groups probably arise from the small decrease in ion concentration, and consequently disordered positions for the ion.
Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA)*d(TGCGCG) in two crystal forms.,Thiyagarajan S, Rajan SS, Gautham N Nucleic Acids Res. 2004 Nov 8;32(19):5945-53. Print 2004. PMID:15534365<ref>PMID:15534365</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1xa2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Gautham, N]]
[[Category: Gautham N]]
[[Category: Rajan, S S]]
[[Category: Rajan SS]]
[[Category: Thiyagarajan, S]]
[[Category: Thiyagarajan S]]
[[Category: Dna]]
[[Category: Double helix]]
[[Category: Z-dna]]

Latest revision as of 11:48, 14 February 2024

Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA).d(TGCGCG) in two crystal formsCobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA).d(TGCGCG) in two crystal forms

Structural highlights

1xa2 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.71Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

1xa2, resolution 1.71Å

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