1x8s: Difference between revisions

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-[[Image:1x8s.jpg|left|200px]]<br /><applet load="1x8s" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1x8s, resolution 2.503&Aring;" />
-'''Structure of the Par-6 PDZ domain with a Pals1 internal ligand'''<br />
-==Overview==
+==Structure of the Par-6 PDZ domain with a Pals1 internal ligand==
-PDZ protein interaction domains are typically selective for C-terminal ligands, but non-C-terminal, 'internal' ligands have also been identified. The PDZ domain from the cell polarity protein Par-6 binds C-terminal ligands and an internal sequence from the protein Pals1/Stardust. The structure of the Pals1-Par-6 PDZ complex reveals that the PDZ ligand-binding site is deformed to allow for internal binding. Whereas binding of the Rho GTPase Cdc42 to a CRIB domain adjacent to the Par-6 PDZ regulates binding of C-terminal ligands, the conformational change that occurs upon binding of Pals1 renders its binding independent of Cdc42. These results suggest a mechanism by which the requirement for a C terminus can be readily bypassed by PDZ ligands and reveal a complex set of cooperative and competitive interactions in Par-6 that are likely to be important for cell polarity regulation.
+<StructureSection load='1x8s' size='340' side='right'caption='[[1x8s]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1x8s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X8S FirstGlance]. <br>
-X8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8S OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.503&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8s OCA], [https://pdbe.org/1x8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x8s RCSB], [https://www.ebi.ac.uk/pdbsum/1x8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x8s ProSAT]</span></td></tr>
-==Reference==
+</table>
-Internal recognition through PDZ domain plasticity in the Par-6-Pals1 complex., Penkert RR, DiVittorio HM, Prehoda KE, Nat Struct Mol Biol. 2004 Nov;11(11):1122-7. Epub 2004 Oct 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15475968 15475968]
+== Function ==
+[https://www.uniprot.org/uniprot/O97111_DROME O97111_DROME]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/1x8s_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x8s ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
 [[Category: Drosophila melanogaster]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: DiVittorio, H M.]]
+[[Category: DiVittorio HM]]
-[[Category: Penkert, R R.]]
+[[Category: Penkert RR]]
-[[Category: Prehoda, K E.]]
+[[Category: Prehoda KE]]
-[[Category: cell cycle]]
-[[Category: par-6]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:14 2008''