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==GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)==
==GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)==
<StructureSection load='1vqb' size='340' side='right' caption='[[1vqb]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='1vqb' size='340' side='right'caption='[[1vqb]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1vqb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpf1 Bpf1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bgh 1bgh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VQB FirstGlance]. <br>
<table><tr><td colspan='2'>[[1vqb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_f1 Enterobacteria phage f1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bgh 1bgh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VQB FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vqb OCA], [http://pdbe.org/1vqb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vqb RCSB], [http://www.ebi.ac.uk/pdbsum/1vqb PDBsum]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vqb OCA], [https://pdbe.org/1vqb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vqb RCSB], [https://www.ebi.ac.uk/pdbsum/1vqb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vqb ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/G5P_BPF1 G5P_BPF1]] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).  
[https://www.uniprot.org/uniprot/G5P_BPF1 G5P_BPF1] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vq/1vqb_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vq/1vqb_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
Line 17: Line 19:
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vqb ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vqb ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47--&gt;methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 A resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein-single-stranded DNA complex.
Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein.,Skinner MM, Zhang H, Leschnitzer DH, Guan Y, Bellamy H, Sweet RM, Gray CW, Konings RN, Wang AH, Terwilliger TC Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2071-5. PMID:8134350<ref>PMID:8134350</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1vqb" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bpf1]]
[[Category: Enterobacteria phage f1]]
[[Category: Bellamy, H]]
[[Category: Large Structures]]
[[Category: Gray, C W]]
[[Category: Bellamy H]]
[[Category: Guan, Y]]
[[Category: Gray CW]]
[[Category: Konings, R N.H]]
[[Category: Guan Y]]
[[Category: Leschnitzer, D H]]
[[Category: Konings RNH]]
[[Category: Skinner, M M]]
[[Category: Leschnitzer DH]]
[[Category: Sweet, R M]]
[[Category: Skinner MM]]
[[Category: Terwilliger, T C]]
[[Category: Sweet RM]]
[[Category: Wang, A H.J]]
[[Category: Terwilliger TC]]
[[Category: Zhang, H]]
[[Category: Wang AH-J]]
[[Category: Dna-binding protein]]
[[Category: Zhang H]]
[[Category: Gene v]]

Latest revision as of 11:46, 14 February 2024

GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)

Structural highlights

1vqb is a 1 chain structure with sequence from Enterobacteria phage f1. This structure supersedes the now removed PDB entry 1bgh. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G5P_BPF1 Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1vqb, resolution 1.80Å

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OCA