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[[Image:1vqb.jpg|left|200px]]<br /><applet load="1vqb" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1vqb, resolution 1.8&Aring;" />
'''GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)'''<br />


==Overview==
==GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)==
The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47--&gt;methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 A resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein-single-stranded DNA complex.
<StructureSection load='1vqb' size='340' side='right'caption='[[1vqb]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1vqb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_f1 Enterobacteria phage f1]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bgh 1bgh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VQB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vqb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vqb OCA], [https://pdbe.org/1vqb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vqb RCSB], [https://www.ebi.ac.uk/pdbsum/1vqb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vqb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G5P_BPF1 G5P_BPF1] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vq/1vqb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vqb ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1VQB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_f1 Bacteriophage f1]. This structure supersedes the now removed PDB entry 1BGH. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VQB OCA].
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein., Skinner MM, Zhang H, Leschnitzer DH, Guan Y, Bellamy H, Sweet RM, Gray CW, Konings RN, Wang AH, Terwilliger TC, Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2071-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8134350 8134350]
[[Category: Enterobacteria phage f1]]
[[Category: Bacteriophage f1]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Bellamy H]]
[[Category: Bellamy, H.]]
[[Category: Gray CW]]
[[Category: Gray, C W.]]
[[Category: Guan Y]]
[[Category: Guan, Y.]]
[[Category: Konings RNH]]
[[Category: Konings, R N.H.]]
[[Category: Leschnitzer DH]]
[[Category: Leschnitzer, D H.]]
[[Category: Skinner MM]]
[[Category: Skinner, M M.]]
[[Category: Sweet RM]]
[[Category: Sweet, R M.]]
[[Category: Terwilliger TC]]
[[Category: Terwilliger, T C.]]
[[Category: Wang AH-J]]
[[Category: Wang, A H.J.]]
[[Category: Zhang H]]
[[Category: Zhang, H.]]
[[Category: dna-binding protein]]
[[Category: gene v]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:37:29 2008''

Latest revision as of 11:46, 14 February 2024

GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)

Structural highlights

1vqb is a 1 chain structure with sequence from Enterobacteria phage f1. This structure supersedes the now removed PDB entry 1bgh. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G5P_BPF1 Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1vqb, resolution 1.80Å

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