1u12: Difference between revisions

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[[Image:1u12.gif|left|200px]]


{{Structure
==M. loti cyclic nucleotide binding domain mutant==
|PDB= 1u12 |SIZE=350|CAPTION= <scene name='initialview01'>1u12</scene>, resolution 2.70&Aring;
<StructureSection load='1u12' size='340' side='right'caption='[[1u12]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
<table><tr><td colspan='2'>[[1u12]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesorhizobium_japonicum_MAFF_303099 Mesorhizobium japonicum MAFF 303099]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U12 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U12 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u12 OCA], [https://pdbe.org/1u12 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u12 RCSB], [https://www.ebi.ac.uk/pdbsum/1u12 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u12 ProSAT]</span></td></tr>
|RELATEDENTRY=[[1pf0|1PF0]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u12 OCA], [http://www.ebi.ac.uk/pdbsum/1u12 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u12 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/CNGK1_RHILO CNGK1_RHILO] Cyclic nucleotide-regulated potassium channel activated by cAMP.<ref>PMID:15550244</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u1/1u12_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u12 ConSurf].
<div style="clear:both"></div>


'''M. loti cyclic nucleotide binding domain mutant'''
==See Also==
 
*[[Ion channels 3D structures|Ion channels 3D structures]]
 
== References ==
==Overview==
<references/>
Here we describe the initial functional characterization of a cyclic nucleotide regulated ion channel from the bacterium Mesorhizobium loti and present two structures of its cyclic nucleotide binding domain, with and without cAMP. The domains are organized as dimers with the interface formed by the linker regions that connect the nucleotide binding pocket to the pore domain. Together, structural and functional data suggest the domains form two dimers on the cytoplasmic face of the channel. We propose a model for gating in which ligand binding alters the structural relationship within a dimer, directly affecting the position of the adjacent transmembrane helices.
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
1U12 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti_maff303099 Mesorhizobium loti maff303099]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U12 OCA].
[[Category: Mesorhizobium japonicum MAFF 303099]]
 
[[Category: Clayton GM]]
==Reference==
[[Category: Heginbotham L]]
Structural basis of ligand activation in a cyclic nucleotide regulated potassium channel., Clayton GM, Silverman WR, Heginbotham L, Morais-Cabral JH, Cell. 2004 Nov 24;119(5):615-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15550244 15550244]
[[Category: Morais-Cabral JH]]
[[Category: Mesorhizobium loti maff303099]]
[[Category: Silverman WR]]
[[Category: Single protein]]
[[Category: Clayton, G M.]]
[[Category: Heginbotham, L.]]
[[Category: Morais-Cabral, J H.]]
[[Category: Silverman, W R.]]
[[Category: c-helix mutation]]
[[Category: mutant cyclic nucleotide binding domain]]
[[Category: unliganded]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:03:56 2008''

Latest revision as of 11:43, 14 February 2024

M. loti cyclic nucleotide binding domain mutantM. loti cyclic nucleotide binding domain mutant

Structural highlights

1u12 is a 2 chain structure with sequence from Mesorhizobium japonicum MAFF 303099. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CNGK1_RHILO Cyclic nucleotide-regulated potassium channel activated by cAMP.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Clayton GM, Silverman WR, Heginbotham L, Morais-Cabral JH. Structural basis of ligand activation in a cyclic nucleotide regulated potassium channel. Cell. 2004 Nov 24;119(5):615-27. PMID:15550244 doi:10.1016/j.cell.2004.10.030

1u12, resolution 2.70Å

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