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==Y33H Mutant of Homing endonuclease I-CreI==
==Y33H Mutant of Homing endonuclease I-CreI==
<StructureSection load='1u0d' size='340' side='right' caption='[[1u0d]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
<StructureSection load='1u0d' size='340' side='right'caption='[[1u0d]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1u0d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0D FirstGlance]. <br>
<table><tr><td colspan='2'>[[1u0d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0D FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1u0c|1u0c]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0d OCA], [http://pdbe.org/1u0d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u0d RCSB], [http://www.ebi.ac.uk/pdbsum/1u0d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0d ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0d OCA], [https://pdbe.org/1u0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0d RCSB], [https://www.ebi.ac.uk/pdbsum/1u0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0d ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.  
[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0d ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0d ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites. Due to their biological function and high level of target specificity, these enzymes are under intense investigation as tools for gene targeting. These studies require that naturally occurring enzymes be redesigned to recognize novel target sites. Here, we report studies in which the homodimeric LAGLIDADG homing endonuclease I-CreI is altered at individual side-chains corresponding to contact points to distinct base-pairs in its target site. The resulting enzyme constructs drive specific elimination of selected DNA targets in vivo and display shifted specificities of DNA binding and cleavage in vitro. Crystal structures of two of these constructs demonstrate that substitution of individual side-chain/DNA contact patterns can occur with almost no structural deformation or rearrangement of the surrounding complex, facilitating an isolated, modular redesign strategy for homing endonuclease activity and specificity.
Isolation and characterization of new homing endonuclease specificities at individual target site positions.,Sussman D, Chadsey M, Fauce S, Engel A, Bruett A, Monnat R Jr, Stoddard BL, Seligman LM J Mol Biol. 2004 Sep 3;342(1):31-41. PMID:15313605<ref>PMID:15313605</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1u0d" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chlre]]
[[Category: Chlamydomonas reinhardtii]]
[[Category: Bruett, A]]
[[Category: Large Structures]]
[[Category: Chadsey, M]]
[[Category: Bruett A]]
[[Category: Engel, A]]
[[Category: Chadsey M]]
[[Category: Fauce, S]]
[[Category: Engel A]]
[[Category: Monnat, R]]
[[Category: Fauce S]]
[[Category: Seligman, L M]]
[[Category: Monnat R]]
[[Category: Stoddard, B L]]
[[Category: Seligman LM]]
[[Category: Sussman, D]]
[[Category: Stoddard BL]]
[[Category: Dna endonuclease i-crei]]
[[Category: Sussman D]]
[[Category: Hydrolase-dna complex]]
[[Category: Protein/dna]]

Latest revision as of 11:43, 14 February 2024

Y33H Mutant of Homing endonuclease I-CreIY33H Mutant of Homing endonuclease I-CreI

Structural highlights

1u0d is a 4 chain structure with sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.9Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DNE1_CHLRE Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1u0d, resolution 2.90Å

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OCA
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