1sj7: Difference between revisions

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OCA

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-[[Image:1sj7.gif|left|200px]]<br /><applet load="1sj7" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1sj7, resolution 2.50&Aring;" />
-'''Crystal Structure of Talin Rod 482-655'''<br />
-==Overview==
+==Crystal Structure of Talin Rod 482-655==
-The interaction between the cytoskeletal proteins talin and vinculin plays, a key role in integrin-mediated cell adhesion and migration. We have, determined the crystal structures of two domains from the talin rod, spanning residues 482-789. Talin 482-655, which contains a, vinculin-binding site (VBS), folds into a five-helix bundle whereas talin, 656-789 is a four-helix bundle. We show that the VBS is composed of a, hydrophobic surface spanning five turns of helix 4. All the key side, chains from the VBS are buried and contribute to the hydrophobic core of, the talin 482-655 fold. We demonstrate that the talin 482-655 five-helix, bundle represents an inactive conformation, and mutations that disrupt the, hydrophobic core or deletion of helix 5 are required to induce an active, conformation in which the VBS is exposed. We also report the crystal, structure of the N-terminal vinculin head domain in complex with an, activated form of talin. Activation of the VBS in talin and the, recruitment of vinculin may support the maturation of small integrin/talin, complexes into more stable adhesions.
+<StructureSection load='1sj7' size='340' side='right'caption='[[1sj7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1sj7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SJ7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sj7 OCA], [https://pdbe.org/1sj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sj7 RCSB], [https://www.ebi.ac.uk/pdbsum/1sj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sj7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sj/1sj7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sj7 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-SJ7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with PT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SJ7 OCA].
+*[[Talin 3D structures|Talin 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle., Papagrigoriou E, Gingras AR, Barsukov IL, Bate N, Fillingham IJ, Patel B, Frank R, Ziegler WH, Roberts GC, Critchley DR, Emsley J, EMBO J. 2004 Aug 4;23(15):2942-51. Epub 2004 Jul 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15272303 15272303]
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Single protein]]
+[[Category: Barsukov IL]]
-[[Category: Barsukov, I.L.]]
+[[Category: Critchley DR]]
-[[Category: Critchley, D.R.]]
+[[Category: Emsley J]]
-[[Category: Emsley, J.]]
+[[Category: Gingras AR]]
-[[Category: Gingras, A.R.]]
+[[Category: Papagrigoriou E]]
-[[Category: Papagrigoriou, E.]]
-[[Category: PT]]
-[[Category: structural protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:25:04 2007''