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[[Image:1sdo.jpg|left|200px]]<br /><applet load="1sdo" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1sdo, resolution 1.85&Aring;" />
'''Crystal Structure of Restriction Endonuclease BstYI'''<br />


==Overview==
==Crystal Structure of Restriction Endonuclease BstYI==
We report here the structure of BstYI, an "intermediate" type II restriction endonuclease with overlapping sequence specificities to BamHI and BglII. BstYI, a thermophilic endonuclease, recognizes and cleaves the degenerate hexanucleotide sequence 5'-RGATCY-3' (where R=A or G and Y=C or T), cleaving DNA after the 5'-R on each strand to produce four-base (5') staggered ends. The crystal structure of free BstYI was solved at 1.85A resolution by multi-wavelength anomalous dispersion (MAD) phasing. Comparison with BamHI and BglII reveals a strong structural consensus between all three enzymes mapping to the alpha/beta core domain and residues involved in catalysis. Unexpectedly, BstYI also contains an additional "arm" substructure outside of the core protein, which enables the enzyme to adopt a more compact, intertwined dimer structure compared with BamHI and BglII. This arm substructure may underlie the thermostability of BstYI. We identify putative DNA recognition residues and speculate as to how this enzyme achieves a "relaxed" DNA specificity.
<StructureSection load='1sdo' size='340' side='right'caption='[[1sdo]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1sdo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SDO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SDO FirstGlance]. <br>
1SDO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SDO OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sdo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sdo OCA], [https://pdbe.org/1sdo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sdo RCSB], [https://www.ebi.ac.uk/pdbsum/1sdo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sdo ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of BstYI at 1.85A resolution: a thermophilic restriction endonuclease with overlapping specificities to BamHI and BglII., Townson SA, Samuelson JC, Vanamee ES, Edwards TA, Escalante CR, Xu SY, Aggarwal AK, J Mol Biol. 2004 May 7;338(4):725-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15099740 15099740]
== Function ==
[https://www.uniprot.org/uniprot/Q84AF2_GEOSE Q84AF2_GEOSE]  
__TOC__
</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Aggarwal AK]]
[[Category: Aggarwal, A K.]]
[[Category: Edwards TA]]
[[Category: Edwards, T A.]]
[[Category: Escalante CR]]
[[Category: Escalante, C R.]]
[[Category: Samuelson JC]]
[[Category: Samuelson, J C.]]
[[Category: Townson SA]]
[[Category: Townson, S A.]]
[[Category: Vanamee ES]]
[[Category: Vanamee, E S.]]
[[Category: Xu SY]]
[[Category: Xu, S Y.]]
[[Category: restriction endonuclease]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:00:25 2008''

Latest revision as of 11:29, 14 February 2024

Crystal Structure of Restriction Endonuclease BstYICrystal Structure of Restriction Endonuclease BstYI

Structural highlights

1sdo is a 1 chain structure with sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.85Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q84AF2_GEOSE

1sdo, resolution 1.85Å

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