1sd6: Difference between revisions

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New page: left|200px<br /><applet load="1sd6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sd6, resolution 2.65Å" /> '''Crystal Structure of...
 
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[[Image:1sd6.gif|left|200px]]<br /><applet load="1sd6" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1sd6, resolution 2.65&Aring;" />
'''Crystal Structure of Native MecI at 2.65 A'''<br />


==Overview==
==Crystal Structure of Native MecI at 2.65 A==
The 14-kDa BlaI protein represses the transcription of blaZ, the gene, encoding beta-lactamase. It is homologous to MecI, which regulates the, expression of mecA, the gene encoding the penicillin binding protein, PBP2a. These genes mediate resistance to beta-lactam antibiotics in, staphylococci. Both repressors can bind either bla or mec DNA, promoter-operator sequences. Regulated resistance genes are activated via, receptor-mediated cleavage of the repressors. Cleavage is induced when, beta-lactam antibiotics bind the extramembrane sensor of the, sensor-transducer signaling molecules, BlaR1 or MecR1. The crystal, structures of BlaI from Staphylococcus aureus, both in free form and in, complex with 32 bp of DNA of the mec operator, have been determined to, 2.0- and 2.7-A resolutions, respectively. The structure of MecI, also in, free form and in complex with the bla operator, has been previously, reported. Both repressors form homodimers, with each monomer composed of, an N-terminal DNA binding domain of winged helix-turn-helix topology and a, C-terminal dimerization domain. The structure of BlaI in complex with the, mec operator shows a protein-DNA interface that is conserved between both, mec and bla targets. The recognition helix alpha3 interacts specifically, with the conserved TACA/TGTA DNA binding motif. BlaI and, probably, MecI, dimers bind to opposite faces of the mec DNA double helix in an, up-and-down arrangement, whereas MecI and, probably, BlaI dimers bind to, the same DNA face of bla promoter-operator DNA. This is due to the, different spacing of mec and bla DNA binding sites. Furthermore, the, flexibility of the dimeric proteins may make the C-terminal proteolytic, cleavage site more accessible when the repressors are bound to DNA than, when they are in solution, suggesting that the induction cascade involves, bound rather than free repressor.
<StructureSection load='1sd6' size='340' side='right'caption='[[1sd6]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1sd6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SD6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SD6 FirstGlance]. <br>
1SD6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SD6 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sd6 OCA], [https://pdbe.org/1sd6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sd6 RCSB], [https://www.ebi.ac.uk/pdbsum/1sd6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sd6 ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structures of the BlaI repressor from Staphylococcus aureus and its complex with DNA: insights into transcriptional regulation of the bla and mec operons., Safo MK, Zhao Q, Ko TP, Musayev FN, Robinson H, Scarsdale N, Wang AH, Archer GL, J Bacteriol. 2005 Mar;187(5):1833-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15716455 15716455]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/MECI_STAAU MECI_STAAU] Transcriptional repressor that constitutively blocks the transcription of the gene for the penicillin-binding protein MecA. Binds DNA as a dimer (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sd/1sd6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sd6 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
[[Category: Archer, G.L.]]
[[Category: Archer GL]]
[[Category: Musayev, F.N.]]
[[Category: Musayev FN]]
[[Category: Robinson, H.]]
[[Category: Robinson H]]
[[Category: Safo, M.K.]]
[[Category: Safo MK]]
[[Category: Scarsdale, N.]]
[[Category: Scarsdale N]]
[[Category: Zhao, Q.]]
[[Category: Zhao Q]]
[[Category: b-lactam]]
[[Category: blai]]
[[Category: dna binding protein]]
[[Category: meci]]
[[Category: methicillin]]
[[Category: repressor]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:17:30 2007''

Latest revision as of 11:29, 14 February 2024

Crystal Structure of Native MecI at 2.65 ACrystal Structure of Native MecI at 2.65 A

Structural highlights

1sd6 is a 2 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.65Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MECI_STAAU Transcriptional repressor that constitutively blocks the transcription of the gene for the penicillin-binding protein MecA. Binds DNA as a dimer (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1sd6, resolution 2.65Å

Drag the structure with the mouse to rotate

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