1s7f: Difference between revisions

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-[[Image:1s7f.gif|left|200px]]
- {{Structure
+==RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)==
-|PDB= 1s7f |SIZE=350|CAPTION= <scene name='initialview01'>1s7f</scene>, resolution 2.0&Aring;
+<StructureSection load='1s7f' size='340' side='right'caption='[[1s7f]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=MLA:MALONIC ACID'>MLA</scene>
+<table><tr><td colspan='2'>[[1s7f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S7F FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-|GENE= RimL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 Salmonella typhimurium LT2])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7f OCA], [https://pdbe.org/1s7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s7f RCSB], [https://www.ebi.ac.uk/pdbsum/1s7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s7f ProSAT]</span></td></tr>
+</table>
-'''RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)'''
+== Function ==
+[https://www.uniprot.org/uniprot/Q8ZPC0_SALTY Q8ZPC0_SALTY]
+== Evolutionary Conservation ==
-==Overview==
+[[Image:Consurf_key_small.gif|200px|right]]
-RimL is responsible for converting the prokaryotic ribosomal protein from L12 to L7 by acetylation of its N-terminal amino group. We demonstrate that purified RimL is capable of posttranslationally acetylating L12, exhibiting a V(max) of 21 min(-1). We have also determined the apostructure of RimL from Salmonella typhimurium and its complex with coenzyme A, revealing a homodimeric oligomer with structural similarity to other Gcn5-related N-acetyltransferase superfamily members. A large central trough located at the dimer interface provides sufficient room to bind both L12 N-terminal helices. Structural and biochemical analysis indicates that RimL proceeds by single-step transfer rather than a covalent-enzyme intermediate. This is the first structure of a Gcn5-related N-acetyltransferase family member with demonstrated activity toward a protein N(alpha)-amino group and is a first step toward understanding the molecular basis for N(alpha)acetylation and its function in cellular regulation.
+Check<jmol>
+  <jmolCheckbox>
-==About this Structure==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s7/1s7f_consurf.spt"</scriptWhenChecked>
-S7F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7F OCA].
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==Reference==
+  </jmolCheckbox>
-A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium., Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS, J Biol Chem. 2005 Jun 10;280(23):22108-14. Epub 2005 Apr 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15817456 15817456]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s7f ConSurf].
-[[Category: Salmonella typhimurium lt2]]
+<div style="clear:both"></div>
-[[Category: Single protein]]
+__TOC__
-[[Category: Blanchard, J S.]]
+</StructureSection>
-[[Category: Carvalho, L P.de.]]
+[[Category: Large Structures]]
-[[Category: Roderick, S L.]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
-[[Category: Vetting, M W.]]
+[[Category: Blanchard JS]]
-[[Category: CL]]
+[[Category: Roderick SL]]
-[[Category: MLA]]
+[[Category: Vetting MW]]
-[[Category: acetyltransferase]]
+[[Category: De Carvalho LP]]
-[[Category: alpha-n-protein acetyltransferase]]
-[[Category: coenzyme some]]
-[[Category: gnat]]
-[[Category: l7/l12]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:28 2008''