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[[Image:1rz2.jpg|left|200px]]<br /><applet load="1rz2" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1rz2, resolution 1.60&Aring;" />
'''1.6A crystal structure of the protein BA4783/Q81L49 (similar to sortase B) from Bacillus anthracis.'''<br />


==Overview==
==1.6A crystal structure of the protein BA4783/Q81L49 (similar to sortase B) from Bacillus anthracis.==
Surface proteins attached by sortases to the cell wall envelope of bacterial pathogens play important roles during infection. Sorting and attachment of these proteins is directed by C-terminal signals. Sortase B of S. aureus recognizes a motif NPQTN, cleaves the polypeptide after the Thr residue, and attaches the protein to pentaglycine cross-bridges. Sortase B of B. anthracis is thought to recognize the NPKTG motif, and attaches surface proteins to m-diaminopimelic acid cross-bridges. We have determined crystal structure of sortase B from B. anthracis and S. aureus at 1.6 and 2.0 A resolutions, respectively. These structures show a beta-barrel fold with alpha-helical elements on its outside, a structure thus far exclusive to the sortase family. A putative active site located on the edge of the beta-barrel is comprised of a Cys-His-Asp catalytic triad and presumably faces the bacterial cell surface. A putative binding site for the sorting signal is located nearby.
<StructureSection load='1rz2' size='340' side='right'caption='[[1rz2]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1rz2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._Ames Bacillus anthracis str. Ames]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZ2 FirstGlance]. <br>
1RZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_str._ames Bacillus anthracis str. ames]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ2 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz2 OCA], [https://pdbe.org/1rz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rz2 RCSB], [https://www.ebi.ac.uk/pdbsum/1rz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz2 ProSAT], [https://www.topsan.org/Proteins/MCSG/1rz2 TOPSAN]</span></td></tr>
==Reference==
</table>
Structures of sortase B from Staphylococcus aureus and Bacillus anthracis reveal catalytic amino acid triad in the active site., Zhang R, Wu R, Joachimiak G, Mazmanian SK, Missiakas DM, Gornicki P, Schneewind O, Joachimiak A, Structure. 2004 Jul;12(7):1147-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15242591 15242591]
== Function ==
[[Category: Bacillus anthracis str. ames]]
[https://www.uniprot.org/uniprot/A0A6L8PZR0_BACAN A0A6L8PZR0_BACAN]  
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Gornicki, P.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Joachimiak, A.]]
Check<jmol>
[[Category: MCSG, Midwest Center for Structural Genomics.]]
  <jmolCheckbox>
[[Category: Wu, R.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rz2_consurf.spt"</scriptWhenChecked>
[[Category: Zhang, R.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: b. anthracis]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: mcsg]]
  </jmolCheckbox>
[[Category: midwest center for structural genomics]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rz2 ConSurf].
[[Category: protein structure initiative]]
<div style="clear:both"></div>
[[Category: psi]]
__TOC__
[[Category: sortase b protein]]
</StructureSection>
[[Category: structural genomics]]
[[Category: Bacillus anthracis str. Ames]]
 
[[Category: Large Structures]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:07 2008''
[[Category: Gornicki P]]
[[Category: Joachimiak A]]
[[Category: Wu R]]
[[Category: Zhang R]]

Latest revision as of 11:27, 14 February 2024

1.6A crystal structure of the protein BA4783/Q81L49 (similar to sortase B) from Bacillus anthracis.1.6A crystal structure of the protein BA4783/Q81L49 (similar to sortase B) from Bacillus anthracis.

Structural highlights

1rz2 is a 1 chain structure with sequence from Bacillus anthracis str. Ames. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

A0A6L8PZR0_BACAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1rz2, resolution 1.60Å

Drag the structure with the mouse to rotate

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OCA
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