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[[Image:1ryn.jpg|left|200px]]<br /><applet load="1ryn" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1ryn, resolution 1.75&Aring;" />
'''Structure of the Chloroplast Group II Intron Splicing Factor CRS2'''<br />


==Overview==
==Structure of the Chloroplast Group II Intron Splicing Factor CRS2==
Chloroplast RNA splicing 2 (CRS2) is a nuclear-encoded protein required for the splicing of nine group II introns in maize chloroplasts. CRS2 functions in the context of splicing complexes that include one of two CRS2-associated factors (CAF1 and CAF2). The CRS2-CAF1 and CRS2-CAF2 complexes are required for the splicing of different subsets of CRS2-dependent introns, and they bind tightly and specifically to their genetically defined intron targets in vivo. The CRS2 amino acid sequence is closely related to those of bacterial peptidyl-tRNA hydrolases (PTHs). To identify the structural differences between CRS2 and bacterial PTHs responsible for CRS2's gains of CAF binding and intron splicing functions, we determined the structure of CRS2 by X-ray crystallography. The fold of CRS2 is the same as that of Escherichia coli PTH, but CRS2 has two surfaces that differ from the corresponding surfaces in PTH. One of these is more hydrophobic in CRS2 than in PTH. Site-directed mutagenesis of this surface blocked CRS2-CAF complex formation, indicating that it is the CAF binding site. The CRS2 surface corresponding to the putative tRNA binding face of PTH is considerably more basic than in PTH, suggesting that CRS2 interacts with group II intron substrates via this surface. Both the sequence and the structural context of the amino acid residues essential for peptidyl-tRNA hydrolase activity are conserved in CRS2, yet expression of CRS2 is incapable of rescuing a pth(ts)E.coli strain.
<StructureSection load='1ryn' size='340' side='right'caption='[[1ryn]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1ryn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RYN FirstGlance]. <br>
1RYN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYN OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ryn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ryn OCA], [https://pdbe.org/1ryn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ryn RCSB], [https://www.ebi.ac.uk/pdbsum/1ryn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ryn ProSAT]</span></td></tr>
==Reference==
</table>
Structural analysis of the group II intron splicing factor CRS2 yields insights into its protein and RNA interaction surfaces., Ostheimer GJ, Hadjivassiliou H, Kloer DP, Barkan A, Matthews BW, J Mol Biol. 2005 Jan 7;345(1):51-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15567410 15567410]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/CRS2_MAIZE CRS2_MAIZE] Required for the splicing of group IIB introns in chloroplasts. Forms complexes with either CAF1 or CAF2 which, in turn, interact with RNA and confer intron specificity of the splicing particles. Has no peptidyl-tRNA hydrolase activity.<ref>PMID:11179231</ref> <ref>PMID:9090875</ref> <ref>PMID:10481026</ref> <ref>PMID:12881426</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ry/1ryn_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ryn ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Zea mays]]
[[Category: Zea mays]]
[[Category: Barkan, A.]]
[[Category: Barkan A]]
[[Category: Matthews, B W.]]
[[Category: Matthews BW]]
[[Category: Ostheimer, G J.]]
[[Category: Ostheimer GJ]]
[[Category: alpha-beta]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:03 2008''

Latest revision as of 11:26, 14 February 2024

Structure of the Chloroplast Group II Intron Splicing Factor CRS2Structure of the Chloroplast Group II Intron Splicing Factor CRS2

Structural highlights

1ryn is a 1 chain structure with sequence from Zea mays. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.75Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CRS2_MAIZE Required for the splicing of group IIB introns in chloroplasts. Forms complexes with either CAF1 or CAF2 which, in turn, interact with RNA and confer intron specificity of the splicing particles. Has no peptidyl-tRNA hydrolase activity.[1] [2] [3] [4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Jenkins BD, Barkan A. Recruitment of a peptidyl-tRNA hydrolase as a facilitator of group II intron splicing in chloroplasts. EMBO J. 2001 Feb 15;20(4):872-9. PMID:11179231 doi:http://dx.doi.org/10.1093/emboj/20.4.872
  2. ↑ Jenkins BD, Kulhanek DJ, Barkan A. Nuclear mutations that block group II RNA splicing in maize chloroplasts reveal several intron classes with distinct requirements for splicing factors. Plant Cell. 1997 Mar;9(3):283-96. PMID:9090875 doi:http://dx.doi.org/10.1105/tpc.9.3.283
  3. ↑ Vogel J, Borner T, Hess WR. Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants. Nucleic Acids Res. 1999 Oct 1;27(19):3866-74. PMID:10481026
  4. ↑ Ostheimer GJ, Williams-Carrier R, Belcher S, Osborne E, Gierke J, Barkan A. Group II intron splicing factors derived by diversification of an ancient RNA-binding domain. EMBO J. 2003 Aug 1;22(15):3919-29. PMID:12881426 doi:http://dx.doi.org/10.1093/emboj/cdg372

1ryn, resolution 1.75Å

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