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[[Image:1rkd.gif|left|200px]]<br /><applet load="1rkd" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1rkd, resolution 1.84&Aring;" />
'''E. COLI RIBOKINASE COMPLEXED WITH RIBOSE AND ADP'''<br />


==Overview==
==E. COLI RIBOKINASE COMPLEXED WITH RIBOSE AND ADP==
BACKGROUND: D-ribose must be phosphorylated at O5' before it can be used in either anabolism or catabolism. This reaction is catalysed by ribokinase and requires the presence of ATP and magnesium. Ribokinase is a member of a family of carbohydrate kinases of previously unknown structure. RESULTS: The crystal structure of ribokinase from Escherichia coli in complex with ribose and dinucleotide was determined at 1.84 A resolution by multiple isomorphous replacement. There is one 33 kDa monomer of ribokinase in the asymmetric unit but the protein forms a dimer around a crystallographic twofold axis. Each subunit consists of a central alpha/beta unit, with a new type of nucleotide-binding fold, and a distinct beta sheet that forms a lid over the ribose-binding site. Contact between subunits involves orthogonal packing of beta sheets, in a novel dimer interaction that we call a beta clasp. CONCLUSIONS: Inspection of the complex indicates that ribokinase utilises both a catalytic base for activation of the ribose in nucleophilic attack and an anion hole that stabilises the transition state during phosphoryl transfer. The structure suggests an ordered reaction mechanism, similar to those proposed for other carbohydrate kinases that probably involves conformational changes. We propose that the beta-clasp structure acts as a lid, closing and opening upon binding and release of ribose. From these observations, an understanding of the structure and catalytic mechanism of related sugar kinases can be obtained.
<StructureSection load='1rkd' size='340' side='right'caption='[[1rkd]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1rkd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RKD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=RIB:RIBOSE'>RIB</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rkd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rkd OCA], [https://pdbe.org/1rkd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rkd RCSB], [https://www.ebi.ac.uk/pdbsum/1rkd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rkd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RBSK_ECOLI RBSK_ECOLI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rk/1rkd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rkd ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1RKD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=RIB:'>RIB</scene>, <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribokinase Ribokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.15 2.7.1.15] Known structural/functional Sites: <scene name='pdbsite=AB1:Residues+Making+Direct+H+Bonds+w.+Adp'>AB1</scene>, <scene name='pdbsite=AB2:Residues+Making+Water-Mediated+H+Bonds+w.+Adp'>AB2</scene>, <scene name='pdbsite=AB3:Residues+Within+Van+Der+Waals+Distance+Of+Adp'>AB3</scene>, <scene name='pdbsite=RB1:Residues+Making+Direct+H+Bonds+w.+Ribose'>RB1</scene>, <scene name='pdbsite=RB2:Residues+Making+Water-Mediated+H+Bonds+w.+Ribose'>RB2</scene> and <scene name='pdbsite=RB3:Residues+Within+Van+Der+Waals+Distance+Of+Ribose'>RB3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKD OCA].
*[[Ribokinase 3D structures|Ribokinase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8 A resolution: insights into a new family of kinase structures., Sigrell JA, Cameron AD, Jones TA, Mowbray SL, Structure. 1998 Feb 15;6(2):183-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9519409 9519409]
[[Category: Escherichia coli K-12]]
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Ribokinase]]
[[Category: Cameron AD]]
[[Category: Single protein]]
[[Category: Jones TA]]
[[Category: Cameron, A D.]]
[[Category: Mowbray SL]]
[[Category: Jones, T A.]]
[[Category: Sigrell JA]]
[[Category: Mowbray, S L.]]
[[Category: Sigrell, J A.]]
[[Category: ADP]]
[[Category: PO4]]
[[Category: RIB]]
[[Category: carbohydrate kinase]]
[[Category: nucleotide binding]]
[[Category: ribose]]
[[Category: transferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:56 2008''

Latest revision as of 11:23, 14 February 2024

E. COLI RIBOKINASE COMPLEXED WITH RIBOSE AND ADPE. COLI RIBOKINASE COMPLEXED WITH RIBOSE AND ADP

Structural highlights

1rkd is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.84Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RBSK_ECOLI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1rkd, resolution 1.84Å

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