1rj9: Difference between revisions

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-[[Image:1rj9.gif|left|200px]]<br /><applet load="1rj9" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1rj9, resolution 1.9&Aring;" />
-'''Structure of the heterodimer of the conserved GTPase domains of the Signal Recognition Particle (Ffh) and Its Receptor (FtsY)'''<br />
-==Overview==
+==Structure of the heterodimer of the conserved GTPase domains of the Signal Recognition Particle (Ffh) and Its Receptor (FtsY)==
-Signal sequences target proteins for secretion from cells or for integration into cell membranes. As nascent proteins emerge from the ribosome, signal sequences are recognized by the signal recognition particle (SRP), which subsequently associates with its receptor (SR). In this complex, the SRP and SR stimulate each other's GTPase activity, and GTP hydrolysis ensures unidirectional targeting of cargo through a translocation pore in the membrane. To define the mechanism of reciprocal activation, we determined the 1.9 A structure of the complex formed between these two GTPases. The two partners form a quasi-two-fold symmetrical heterodimer. Biochemical analysis supports the importance of the extensive interaction surface. Complex formation aligns the two GTP molecules in a symmetrical, composite active site, and the 3'OH groups are essential for association, reciprocal activation and catalysis. This unique circle of twinned interactions is severed twice on hydrolysis, leading to complex dissociation after cargo delivery.
+<StructureSection load='1rj9' size='340' side='right'caption='[[1rj9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1rj9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RJ9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rj9 OCA], [https://pdbe.org/1rj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rj9 RCSB], [https://www.ebi.ac.uk/pdbsum/1rj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rj9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FTSY_THEAQ FTSY_THEAQ] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC) (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rj/1rj9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rj9 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-RJ9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GCP:'>GCP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ9 OCA].
+*[[Signal recognition particle 3D structures|Signal recognition particle 3D structures]]
+*[[Signal recognition particle receptor 3D structures|Signal recognition particle receptor 3D structures]]
-==Reference==
+__TOC__
-Substrate twinning activates the signal recognition particle and its receptor., Egea PF, Shan SO, Napetschnig J, Savage DF, Walter P, Stroud RM, Nature. 2004 Jan 15;427(6971):215-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14724630 14724630]
+</StructureSection>
-[[Category: Protein complex]]
+[[Category: Large Structures]]
 [[Category: Thermus aquaticus]]
-[[Category: Egea, P F.]]
+[[Category: Egea PF]]
-[[Category: Napetschnig, J.]]
+[[Category: Napetschnig J]]
-[[Category: Savage, D F.]]
+[[Category: Savage DF]]
-[[Category: Shan, S O.]]
+[[Category: Shan SO]]
-[[Category: Stroud, R M.]]
+[[Category: Stroud RM]]
-[[Category: Walter, P.]]
+[[Category: Walter P]]
-[[Category: GCP]]
-[[Category: MG]]
-[[Category: heterodimer]]
-[[Category: nucleotide twinning]]
-[[Category: protein-protein complex]]
-[[Category: srp-gtpase domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:24 2008''