1rf5: Difference between revisions

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<StructureSection load='1rf5' size='340' side='right'caption='[[1rf5]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='1rf5' size='340' side='right'caption='[[1rf5]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1rf5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RF5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RF5 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1rf5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RF5 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rf4|1rf4]], [[1rf6|1rf6]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rf5 OCA], [https://pdbe.org/1rf5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rf5 RCSB], [https://www.ebi.ac.uk/pdbsum/1rf5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rf5 ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1rf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rf5 OCA], [http://pdbe.org/1rf5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rf5 RCSB], [http://www.ebi.ac.uk/pdbsum/1rf5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rf5 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AROA_STRPN AROA_STRPN]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rf5 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rf5 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The shikimate pathway synthesizes aromatic amino acids and other essential metabolites that are necessary for bacteria, plants and fungi to survive. This pathway is not present in vertebrates and therefore represents an attractive target for antibacterial agents. We have successfully crystallized and solved the structure of unliganded, inhibitor-liganded and tetrahedral intermediate (TI)-liganded forms of Streptococcus pneumoniae EPSP synthase. The overall topology of the S. pneumoniae EPSP synthase is similar to that of the Escherichia coli EPSP synthase. In addition, the majority of residues responsible for ligand binding were conserved between the two proteins. TI-liganded structure provides absolute configuration of the C-2 atom from the F-PEP moiety of the enzyme-bound intermediate and also defines key residues responsible for the enzyme reaction. Comparison of the unliganded state and substrate-bound state of the enzyme provides insights into the structural mechanisms involved in dynamic events of ligand binding, domain movement and closure. This structural study of the pathogenic bacteria S. pneumoniae EPSP synthase with inhibitor and TI will provide invaluable information for the design of new-generation antibiotics.
Structural studies of Streptococcus pneumoniae EPSP synthase in unliganded state, tetrahedral intermediate-bound state and S3P-GLP-bound state.,Park H, Hilsenbeck JL, Kim HJ, Shuttleworth WA, Park YH, Evans JN, Kang C Mol Microbiol. 2004 Feb;51(4):963-71. PMID:14763973<ref>PMID:14763973</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1rf5" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[EPSP synthase|EPSP synthase]]
*[[EPSP synthase 3D structures|EPSP synthase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Evans, J N]]
[[Category: Streptococcus pneumoniae]]
[[Category: Hilsenbeck, J L]]
[[Category: Evans JN]]
[[Category: Kang, C]]
[[Category: Hilsenbeck JL]]
[[Category: Kim, H J]]
[[Category: Kang C]]
[[Category: Park, H]]
[[Category: Kim HJ]]
[[Category: Park, Y H]]
[[Category: Park H]]
[[Category: Shuttleworth, W A]]
[[Category: Park YH]]
[[Category: Epsp synthase]]
[[Category: Shuttleworth WA]]
[[Category: Glyphosate]]
[[Category: Pep]]
[[Category: S. pneumoniae]]
[[Category: S3p]]
[[Category: Shikimate pathway]]
[[Category: Transferase]]

Latest revision as of 11:22, 14 February 2024

Structural Studies of Streptococcus pneumoniae EPSP Synthase in Unliganded StateStructural Studies of Streptococcus pneumoniae EPSP Synthase in Unliganded State

Structural highlights

1rf5 is a 4 chain structure with sequence from Streptococcus pneumoniae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AROA_STRPN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1rf5, resolution 2.30Å

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OCA
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