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[[Image:1r8c.jpg|left|200px]]<br /><applet load="1r8c" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1r8c, resolution 1.90&Aring;" />
'''Crystal Structures of an Archaeal Class I CCA-Adding Enzyme and Its Nucleotide'''<br />


==Overview==
==Crystal Structures of an Archaeal Class I CCA-Adding Enzyme and Its Nucleotide==
CCA-adding enzymes catalyze the addition of CCA onto the 3' terminus of immature tRNAs without using a nucleic acid template and have been divided into two classes based on their amino acid sequences. We have determined the crystal structures of a class I CCA-adding enzyme from Archeoglobus fulgidus (AfCCA) and its complexes with ATP, CTP, or UTP. Although it and the class II bacterial Bacillus stearothermophilus CCA enzyme (BstCCA) have similar dimensions and domain architectures (head, neck, body, and tail), only the polymerase domain is structurally homologous. Moreover, the relative orientation of the head domain with respect to the body and tail domains, which appear likely to bind tRNA, differs significantly between the two enzyme classes. Unlike the class II BstCCA, this enzyme binds nucleotides nonspecifically in the absence of bound tRNA. The shape and electrostatic charge distribution of the AfCCA enzyme suggests a model for tRNA binding that accounts for the phosphates that are protected from chemical modification by tRNA binding to AfCCA. The structures of the AfCCA enzyme and the eukaryotic poly(A) polymerase are very similar, implying a close evolutionary relationship between them.
<StructureSection load='1r8c' size='340' side='right'caption='[[1r8c]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1r8c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R8C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R8C FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=UTP:URIDINE+5-TRIPHOSPHATE'>UTP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r8c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r8c OCA], [https://pdbe.org/1r8c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r8c RCSB], [https://www.ebi.ac.uk/pdbsum/1r8c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r8c ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CCA_ARCFU CCA_ARCFU] Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.<ref>PMID:14592988</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r8/1r8c_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r8c ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1R8C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=UTP:'>UTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/tRNA_adenylyltransferase tRNA adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.25 2.7.7.25] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R8C OCA].
*[[CCA-adding enzyme 3D structures|CCA-adding enzyme 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structures of an archaeal class I CCA-adding enzyme and its nucleotide complexes., Xiong Y, Li F, Wang J, Weiner AM, Steitz TA, Mol Cell. 2003 Nov;12(5):1165-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14636575 14636575]
__TOC__
</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: tRNA adenylyltransferase]]
[[Category: Li F]]
[[Category: Li, F.]]
[[Category: Steitz TA]]
[[Category: Steitz, T A.]]
[[Category: Wang J]]
[[Category: Wang, J.]]
[[Category: Weiner AM]]
[[Category: Weiner, A M.]]
[[Category: Xiong Y]]
[[Category: Xiong, Y.]]
[[Category: MN]]
[[Category: NA]]
[[Category: UTP]]
[[Category: cca adding enzyme]]
[[Category: incoming nucleotide]]
[[Category: nucleotidyltransferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:10 2008''

Latest revision as of 11:21, 14 February 2024

Crystal Structures of an Archaeal Class I CCA-Adding Enzyme and Its NucleotideCrystal Structures of an Archaeal Class I CCA-Adding Enzyme and Its Nucleotide

Structural highlights

1r8c is a 1 chain structure with sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CCA_ARCFU Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Okabe M, Tomita K, Ishitani R, Ishii R, Takeuchi N, Arisaka F, Nureki O, Yokoyama S. Divergent evolutions of trinucleotide polymerization revealed by an archaeal CCA-adding enzyme structure. EMBO J. 2003 Nov 3;22(21):5918-27. PMID:14592988 doi:http://dx.doi.org/10.1093/emboj/cdg563

1r8c, resolution 1.90Å

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