1r77: Difference between revisions

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[[Image:1r77.gif|left|200px]]


{{Structure
==Crystal structure of the cell wall targeting domain of peptidylglycan hydrolase ALE-1==
|PDB= 1r77 |SIZE=350|CAPTION= <scene name='initialview01'>1r77</scene>, resolution 1.75&Aring;
<StructureSection load='1r77' size='340' side='right'caption='[[1r77]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1r77]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_capitis Staphylococcus capitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R77 FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Interstitial_collagenase Interstitial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.7 3.4.24.7]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r77 OCA], [https://pdbe.org/1r77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r77 RCSB], [https://www.ebi.ac.uk/pdbsum/1r77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r77 ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''Crystal structure of the cell wall targeting domain of peptidylglycan hydrolase ALE-1'''
[https://www.uniprot.org/uniprot/ALE1_STACP ALE1_STACP] Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
ALE-1, a homologue of lysostaphin, is a peptidoglycan hydrolase that specifically lyses Staphylococcus aureus cell walls by cleaving the pentaglycine linkage between the peptidoglycan chains. Binding of ALE-1 to S. aureus cells through its C-terminal 92 residues, known as the targeting domain, is functionally important for staphylolytic activity. The ALE-1-targeting domain belongs to the SH3b domain family, the prokaryotic counterpart of the eukaryotic SH3 domains. The 1.75 angstroms crystal structure of the targeting domain shows an all-beta fold similar to typical SH3s but with unique features. The structure reveals patches of conserved residues among orthologous targeting domains, forming surface regions that can potentially interact with some common features of the Gram-positive cell wall. ALE-1-targeting domain binding studies employing various bacterial peptidoglycans demonstrate that the length of the interpeptide bridge, as well as the amino acid composition of the peptide, confers the maximum binding of the targeting domain to the staphylococcal peptidoglycan. Truncation of the highly conserved first 9 N-terminal residues results in loss of specificity to S. aureus cell wall-targeting, suggesting that these residues confer specificity to S. aureus cell wall.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r7/1r77_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1R77 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_capitis Staphylococcus capitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R77 OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r77 ConSurf].
Cell wall-targeting domain of glycylglycine endopeptidase distinguishes among peptidoglycan cross-bridges., Lu JZ, Fujiwara T, Komatsuzawa H, Sugai M, Sakon J, J Biol Chem. 2006 Jan 6;281(1):549-58. Epub 2005 Oct 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16257954 16257954]
<div style="clear:both"></div>
[[Category: Interstitial collagenase]]
__TOC__
[[Category: Single protein]]
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus capitis]]
[[Category: Staphylococcus capitis]]
[[Category: Fujiwara, T.]]
[[Category: Fujiwara T]]
[[Category: Komatsuzawa, H.]]
[[Category: Komatsuzawa H]]
[[Category: Lu, J Z.]]
[[Category: Lu JZ]]
[[Category: Sakon, J.]]
[[Category: Sakon J]]
[[Category: Sugai, M.]]
[[Category: Sugai M]]
[[Category: cell wall targeting domain]]
[[Category: glycylglycine endopeptidase]]
[[Category: lysostaphin]]
[[Category: peptidoglycan hydrolase]]
[[Category: sh3b domain]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:47 2008''

Latest revision as of 11:21, 14 February 2024

Crystal structure of the cell wall targeting domain of peptidylglycan hydrolase ALE-1Crystal structure of the cell wall targeting domain of peptidylglycan hydrolase ALE-1

Structural highlights

1r77 is a 2 chain structure with sequence from Staphylococcus capitis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.75Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ALE1_STACP Lyses staphylococcal cells by hydrolyzing the polyglycine interpeptide bridges of the peptidoglycan.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1r77, resolution 1.75Å

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