1r10: Difference between revisions

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-[[Image:1r10.gif|left|200px]]<br /><applet load="1r10" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1r10, resolution 3.00&Aring;" />
-'''Cystic fibrosis transmembrane conductance regulator (CFTR) nucleotide-binding domain one (NBD1) with ATP, I4122 space group'''<br />
-==Overview==
+==Cystic fibrosis transmembrane conductance regulator (CFTR) nucleotide-binding domain one (NBD1) with ATP, I4122 space group==
-Cystic fibrosis transmembrane conductance regulator (CFTR) is an ATP-binding cassette (ABC) transporter that functions as a chloride channel. Nucleotide-binding domain 1 (NBD1), one of two ABC domains in CFTR, also contains sites for the predominant CF-causing mutation and, potentially, for regulatory phosphorylation. We have determined crystal structures for mouse NBD1 in unliganded, ADP- and ATP-bound states, with and without phosphorylation. This NBD1 differs from typical ABC domains in having added regulatory segments, a foreshortened subdomain interconnection, and an unusual nucleotide conformation. Moreover, isolated NBD1 has undetectable ATPase activity and its structure is essentially the same independent of ligand state. Phe508, which is commonly deleted in CF, is exposed at a putative NBD1-transmembrane interface. Our results are consistent with a CFTR mechanism, whereby channel gating occurs through ATP binding in an NBD1-NBD2 nucleotide sandwich that forms upon displacement of NBD1 regulatory segments.
+<StructureSection load='1r10' size='340' side='right'caption='[[1r10]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1r10]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R10 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R10 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r10 OCA], [https://pdbe.org/1r10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r10 RCSB], [https://www.ebi.ac.uk/pdbsum/1r10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r10 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CFTR_MOUSE CFTR_MOUSE] Involved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter. Can inhibit the chloride channel activity of ANO1 (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/1r10_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r10 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-R10 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ATP:'>ATP</scene> and <scene name='pdbligand=ACY:'>ACY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R10 OCA].
+*[[ABC transporter 3D structures|ABC transporter 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Structure of nucleotide-binding domain 1 of the cystic fibrosis transmembrane conductance regulator., Lewis HA, Buchanan SG, Burley SK, Conners K, Dickey M, Dorwart M, Fowler R, Gao X, Guggino WB, Hendrickson WA, Hunt JF, Kearins MC, Lorimer D, Maloney PC, Post KW, Rajashankar KR, Rutter ME, Sauder JM, Shriver S, Thibodeau PH, Thomas PJ, Zhang M, Zhao X, Emtage S, EMBO J. 2004 Jan 28;23(2):282-93. Epub 2003 Dec 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14685259 14685259]
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Single protein]]
+[[Category: Buchanan SG]]
-[[Category: Buchanan, S G.]]
+[[Category: Burley SK]]
-[[Category: Burley, S K.]]
+[[Category: Conners K]]
-[[Category: Conners, K.]]
+[[Category: Dickey M]]
-[[Category: Dickey, M.]]
+[[Category: Dorwart M]]
-[[Category: Dorwart, M.]]
+[[Category: Fowler R]]
-[[Category: Fowler, R.]]
+[[Category: Gao X]]
-[[Category: Gao, X.]]
+[[Category: Guggino WB]]
-[[Category: Guggino, W B.]]
+[[Category: Hendrickson WA]]
-[[Category: Hendrickson, W A.]]
+[[Category: Lewis HA]]
-[[Category: Lewis, H A.]]
-[[Category: ACY]]
-[[Category: ATP]]
-[[Category: MG]]
-[[Category: abc transporter nucleotide binding domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:45:53 2008''