1qzh: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1qzh.gif|left|200px]]<br /><applet load="1qzh" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1qzh, resolution 2.4&Aring;" />
-'''Crystal structure of Pot1 (protection of telomere)- ssDNA complex'''<br />
-==Overview==
+==Crystal structure of Pot1 (protection of telomere)- ssDNA complex==
-Telomeres, specialized protein-DNA complexes that cap the ends of linear, chromosomes, are essential for protecting chromosomes from degradation and, end-to-end fusions. The Pot1 (protection of telomeres 1) protein is a, widely distributed eukaryotic end-capping protein, having been identified, in fission yeast, microsporidia, plants and animals. Schizosaccharomyces, pombe Pot1p is essential for telomere maintenance, and human POT1 has been, implicated in telomerase regulation. Pot1 binds telomeric single-stranded, DNA (ssDNA) with exceptionally high sequence specificity, the molecular, basis of which has been unknown. Here we describe the 1.9-A-resolution, crystal structure of the amino-terminal DNA-binding domain of S. pombe, Pot1p complexed with ssDNA. The protein adopts an, oligonucleotide/oligosaccharide-binding (OB) fold with two loops that, protrude to form a clamp for ssDNA binding. The structure explains the, sequence specificity of binding: in the context of the Pot1 protein, DNA, self-recognition involving base-stacking and unusual G-T base pairs, compacts the DNA. Any sequence change disrupts the ability of the DNA to, form this structure, preventing it from contacting the array of protein, hydrogen-bonding groups. The structure also explains how Pot1p avoids, binding the vast excess of RNA in the nucleus.
+<StructureSection load='1qzh' size='340' side='right'caption='[[1qzh]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1qzh]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZH FirstGlance]. <br>
-QZH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QZH OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qzh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzh OCA], [https://pdbe.org/1qzh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qzh RCSB], [https://www.ebi.ac.uk/pdbsum/1qzh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzh ProSAT]</span></td></tr>
-==Reference==
+</table>
-DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA., Lei M, Podell ER, Baumann P, Cech TR, Nature. 2003 Nov 13;426(6963):198-203. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14614509 14614509]
+== Function ==
+[https://www.uniprot.org/uniprot/POT1_SCHPO POT1_SCHPO] Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.<ref>PMID:11349150</ref> <ref>PMID:12463756</ref> <ref>PMID:18535244</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qzh_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzh ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Schizosaccharomyces pombe]]
-[[Category: Single protein]]
+[[Category: Baumann P]]
-[[Category: Baumann, P.]]
+[[Category: Cech TR]]
-[[Category: Cech, T.R.]]
+[[Category: Lei M]]
-[[Category: Lei, M.]]
+[[Category: Podell ER]]
-[[Category: Podell, E.R.]]
-[[Category: protein-dna complex]]
-[[Category: single-stranded telomeric dna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:08:50 2007''