1qxy: Difference between revisions

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-[[Image:1qxy.gif|left|200px]]<br /><applet load="1qxy" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1qxy, resolution 1.04&Aring;" />
-'''Crystal structure of S. aureus methionine aminopeptidase in complex with a ketoheterocycle 618'''<br />
-==Overview==
+==Crystal structure of S. aureus methionine aminopeptidase in complex with a ketoheterocycle 618==
-High-resolution crystal structures of Staphylococcus aureus methionine aminopeptidase I in complex with various keto heterocycles and aminoketones were determined, and the intermolecular ligand interactions with the enzyme are reported. The compounds are effective inhibitors of the S. aureus enzyme because of the formation of an uncleavable tetrahedral intermediate upon binding. The electron densities unequivocally show the enzyme-catalyzed transition-state analogue mimicking that for amide bond hydrolysis of substrates.
+<StructureSection load='1qxy' size='340' side='right'caption='[[1qxy]], [[Resolution|resolution]] 1.04&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1qxy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QXY FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.04&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=M2C:(2S)-2-AMINO-4-(METHYLSULFANYL)-1-PYRIDIN-2-YLBUTANE-1,1-DIOL'>M2C</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxy OCA], [https://pdbe.org/1qxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qxy RCSB], [https://www.ebi.ac.uk/pdbsum/1qxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qxy ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MAP1_STAAM MAP1_STAAM]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qx/1qxy_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qxy ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-QXY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=CO:'>CO</scene>, <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=M2C:'>M2C</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Methionyl_aminopeptidase Methionyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.18 3.4.11.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXY OCA].
+*[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structures of Staphylococcusaureus methionine aminopeptidase complexed with keto heterocycle and aminoketone inhibitors reveal the formation of a tetrahedral intermediate., Douangamath A, Dale GE, D'Arcy A, Almstetter M, Eckl R, Frutos-Hoener A, Henkel B, Illgen K, Nerdinger S, Schulz H, Mac Sweeney A, Thormann M, Treml A, Pierau S, Wadman S, Oefner C, J Med Chem. 2004 Mar 11;47(6):1325-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14998322 14998322]
+[[Category: Large Structures]]
-[[Category: Methionyl aminopeptidase]]
-[[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
-[[Category: Arcy, A D.]]
+[[Category: D'Arcy A]]
-[[Category: Dale, G E.]]
+[[Category: Dale GE]]
-[[Category: Douangamath, A.]]
+[[Category: Douangamath A]]
-[[Category: Oefner, C.]]
+[[Category: Oefner C]]
-[[Category: ACT]]
-[[Category: CO]]
-[[Category: M2C]]
-[[Category: pita bread fold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:53 2008''