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New page: left|200px<br /><applet load="1qtf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qtf, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1qtf.gif|left|200px]]<br /><applet load="1qtf" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1qtf, resolution 2.4&Aring;" />
'''CRYSTAL STRUCTURE OF EXFOLIATIVE TOXIN B'''<br />


==Overview==
==CRYSTAL STRUCTURE OF EXFOLIATIVE TOXIN B==
The exfoliative toxins (ETs) cause staphylococcal scalded skin syndrome, a, disease characterized by specific separation of layers of the skin., Evidence suggests that the toxins act as serine proteases, though the, specific substrate and mode of action are not known for certain. The, crystal structure of exfoliative toxin A (ETA) was reported earlier and, shown to be similar to that of the chymotrypsin-like serine proteases., Here, we report the 2.4 A resolution crystal structure of the other, exfoliative toxin, ETB, which is 40% identical to ETA. The overall, structures of ETA and ETB are similar including the positions of key, residues within the active site. The structure of ETB supports the, previous findings that the ETs are serine proteases that cleave substrates, after glutamic acid residues. In this study we also discuss a number of, structural differences including a large 14 residue loop insertion which, may be a key feature involved in the differing biological properties of, the ETs, particularly the pyrogenic and lethal activities of ETB not, shared by ETA.
<StructureSection load='1qtf' size='340' side='right'caption='[[1qtf]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1qtf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QTF FirstGlance]. <br>
1QTF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QTF OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qtf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qtf OCA], [https://pdbe.org/1qtf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qtf RCSB], [https://www.ebi.ac.uk/pdbsum/1qtf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qtf ProSAT]</span></td></tr>
==Reference==
</table>
The crystal structure of exfoliative toxin B: a superantigen with enzymatic activity., Vath GM, Earhart CA, Monie DD, Iandolo JJ, Schlievert PM, Ohlendorf DH, Biochemistry. 1999 Aug 10;38(32):10239-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10441117 10441117]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/ETB_STAAU ETB_STAAU] Has serine protease-like properties and binds to the skin protein profilaggrin. Cleaves substrates after acidic residues. Exfoliative toxins cause impetigous diseases commonly referred as staphylococcal scalded skin syndrome (SSSS).<ref>PMID:2117445</ref> <ref>PMID:2384148</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qt/1qtf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qtf ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
[[Category: Earhart, C.A.]]
[[Category: Earhart CA]]
[[Category: Monie, D.D.]]
[[Category: Monie DD]]
[[Category: Ohlendorf, D.H.]]
[[Category: Ohlendorf DH]]
[[Category: Schlievert, P.M.]]
[[Category: Schlievert PM]]
[[Category: Vath, G.M.]]
[[Category: Vath GM]]
[[Category: serine protease]]
[[Category: superantigen]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:58:12 2007''

Latest revision as of 11:17, 14 February 2024

CRYSTAL STRUCTURE OF EXFOLIATIVE TOXIN BCRYSTAL STRUCTURE OF EXFOLIATIVE TOXIN B

Structural highlights

1qtf is a 1 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ETB_STAAU Has serine protease-like properties and binds to the skin protein profilaggrin. Cleaves substrates after acidic residues. Exfoliative toxins cause impetigous diseases commonly referred as staphylococcal scalded skin syndrome (SSSS).[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Bailey CJ, Smith TP. The reactive serine residue of epidermolytic toxin A. Biochem J. 1990 Jul 15;269(2):535-7. PMID:2117445
  2. ↑ Dancer SJ, Garratt R, Saldanha J, Jhoti H, Evans R. The epidermolytic toxins are serine proteases. FEBS Lett. 1990 Jul 30;268(1):129-32. PMID:2384148

1qtf, resolution 2.40Å

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