1qrr: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1qrr.gif|left|200px]]<br /><applet load="1qrr" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1qrr, resolution 1.6&Aring;" />
-'''CRYSTAL STRUCTURE OF SQD1 PROTEIN COMPLEX WITH NAD AND UDP-GLUCOSE'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF SQD1 PROTEIN COMPLEX WITH NAD AND UDP-GLUCOSE==
-The SQD1 enzyme is believed to be involved in the biosynthesis of the, sulfoquinovosyl headgroup of plant sulfolipids, catalyzing the transfer of, SO(3)(-) to UDP-glucose. We have determined the structure of the complex, of SQD1 from Arabidopsis thaliana with NAD(+) and the putative substrate, UDP-glucose at 1.6-A resolution. Both bound ligands are completely buried, within the binding cleft, along with an internal solvent cavity which is, the likely binding site for the, as yet, unidentified sulfur-donor, substrate. SQD1 is a member of the short-chain dehydrogenase/reductase, (SDR) family of enzymes, and its structure shows a conservation of the SDR, catalytic residues. Among several highly conserved catalytic residues, Thr-145 forms unusually short hydrogen bonds with both susceptible, hydroxyls of UDP-glucose. A His side chain may also be catalytically, important in the sulfonation.
+<StructureSection load='1qrr' size='340' side='right'caption='[[1qrr]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1qrr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QRR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QRR FirstGlance]. <br>
-QRR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with SO4, NAD and UPG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QRR OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UPG:URIDINE-5-DIPHOSPHATE-GLUCOSE'>UPG</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qrr OCA], [https://pdbe.org/1qrr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qrr RCSB], [https://www.ebi.ac.uk/pdbsum/1qrr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qrr ProSAT]</span></td></tr>
-Crystal structure of SQD1, an enzyme involved in the biosynthesis of the plant sulfolipid headgroup donor UDP-sulfoquinovose., Mulichak AM, Theisen MJ, Essigmann B, Benning C, Garavito RM, Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):13097-102. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10557279 10557279]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SQD1_ARATH SQD1_ARATH] Involved in the biosynthesis of sulfolipids found in thylakoid membranes. Converts UDP-glucose and sulfite to the sulfolipid head group precursor UDP-sulfoquinovose.<ref>PMID:11073956</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/1qrr_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qrr ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Arabidopsis thaliana]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Benning, C.]]
+[[Category: Benning C]]
-[[Category: Essigmann, B.]]
+[[Category: Essigmann B]]
-[[Category: Garavito, R.M.]]
+[[Category: Garavito RM]]
-[[Category: Mulichak, A.M.]]
+[[Category: Mulichak AM]]
-[[Category: Theisen, M.J.]]
+[[Category: Theisen MJ]]
-[[Category: NAD]]
-[[Category: SO4]]
-[[Category: UPG]]
-[[Category: rossmann fold]]
-[[Category: sdr homolog]]
-[[Category: short hydrogen bonds]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:55:34 2007''