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[[Image:1qpw.gif|left|200px]]<br /><applet load="1qpw" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1qpw, resolution 1.8&Aring;" />
'''CRYSTAL STRUCTURE DETERMINATION OF PORCINE HEMOGLOBIN AT 1.8A RESOLUTION'''<br />


==Overview==
==CRYSTAL STRUCTURE DETERMINATION OF PORCINE HEMOGLOBIN AT 1.8A RESOLUTION==
To investigate a potential candidate material for making artificial red, blood cells to supplement blood transfusion, the X-ray structure of, porcine haemoglobin at 1.8 A resolution was determined as part of research, towards synthesizing human blood. Porcine haemoglobin was crystallized by, the vapor-diffusion method, producing crystals of dimensions 0.3-0.5 mm, after successive seeding. The crystals belong to the orthorhombic space, group P2(1)2(1)2(1), with unit-cell parameters a = 68.10, b = 72.27, c =, 114.85 A. The initial phase was determined by the molecular-replacement, method, using human oxyhaemoglobin as a model. The final R factor was, 21.1% for 36 820 reflections after validation of 574 water molecules. The, r.m.s. deviations of bond lengths, angles, torsion angles and improper, angles from their ideal values are 0.017 A, 3.0, 20.6 and 1.8 degrees, respectively. The average B factor is 33.63 A(2) for the haemoglobin, molecule and 50.53 A(2) for the water molecules. The structure could be, superimposed on a 2.8 A resolution structure with an r.m.s. difference of, 0.59 A in main-chain atomic positions and 1. 27 A in side-chain atomic, positions. Porcine and human haemoglobins are compared. A tentative model, for artificial blood is proposed based on the complementarity relationship, of the surface charges between haemoglobin and the surrounding cell, membrane.
<StructureSection load='1qpw' size='340' side='right'caption='[[1qpw]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1qpw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QPW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpw OCA], [https://pdbe.org/1qpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qpw RCSB], [https://www.ebi.ac.uk/pdbsum/1qpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qpw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HBA_PIG HBA_PIG] Involved in oxygen transport from the lung to the various peripheral tissues.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/1qpw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qpw ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1QPW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=OXY:'>OXY</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPW OCA].
*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure determination of porcine haemoglobin., Lu TH, Panneerselvam K, Liaw YC, Kan P, Lee CJ, Acta Crystallogr D Biol Crystallogr. 2000 Mar;56(Pt 3):304-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10713517 10713517]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Kan, P.]]
[[Category: Kan P]]
[[Category: Lee, C.J.]]
[[Category: Lee C-J]]
[[Category: Liaw, Y.C.]]
[[Category: Liaw Y-C]]
[[Category: Lu, T.H.]]
[[Category: Lu T-H]]
[[Category: Panneerselvam, K.]]
[[Category: Panneerselvam K]]
[[Category: HEM]]
[[Category: OXY]]
[[Category: artificial human blood]]
[[Category: oxygen transport]]
[[Category: porcine hemoglobin]]
[[Category: x-ray study]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:45:38 2008''

Latest revision as of 11:15, 14 February 2024

CRYSTAL STRUCTURE DETERMINATION OF PORCINE HEMOGLOBIN AT 1.8A RESOLUTIONCRYSTAL STRUCTURE DETERMINATION OF PORCINE HEMOGLOBIN AT 1.8A RESOLUTION

Structural highlights

1qpw is a 4 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HBA_PIG Involved in oxygen transport from the lung to the various peripheral tissues.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1qpw, resolution 1.80Å

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