1q9a: Difference between revisions

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[[Image:1q9a.gif|left|200px]]


{{Structure
==Crystal structure of the sarcin/ricin domain from E.coli 23S rRNA at 1.04 resolution==
|PDB= 1q9a |SIZE=350|CAPTION= <scene name='initialview01'>1q9a</scene>, resolution 1.04&Aring;
<StructureSection load='1q9a' size='340' side='right'caption='[[1q9a]], [[Resolution|resolution]] 1.04&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND=
<table><tr><td colspan='2'>[[1q9a]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q9A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q9A FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.04&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q9a OCA], [https://pdbe.org/1q9a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q9a RCSB], [https://www.ebi.ac.uk/pdbsum/1q9a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q9a ProSAT]</span></td></tr>
}}
</table>
 
__TOC__
'''Crystal structure of the sarcin/ricin domain from E.coli 23S rRNA at 1.04 resolution'''
</StructureSection>
 
[[Category: Large Structures]]
 
[[Category: Beneken J]]
==Overview==
[[Category: Chan YL]]
Bulged-G motifs are ubiquitous internal RNA loops that provide specific recognition sites for proteins and RNAs. To establish the common and distinctive features of the motif we determined the structures of three variants and compared them with related structures. The variants are 27-nt mimics of the sarcin/ricin loop (SRL) from Escherichia coli 23S ribosomal RNA that is an essential part of the binding site for elongation factors (EFs). The wild-type SRL has now been determined at 1.04 A resolution, supplementing data obtained before at 1.11 A and allowing the first calculation of coordinate error for an RNA motif. The other two structures, having a viable (C2658U*G2663A) or a lethal mutation (C2658G*G2663C), were determined at 1.75 and 2.25 A resolution, respectively. Comparisons reveal that bulged-G motifs have a common hydration and geometry, with flexible junctions at flanking structural elements. Six conserved nucleotides preserve the fold of the motif; the remaining seven to nine vary in sequence and alter contacts in both grooves. Differences between accessible functional groups of the lethal mutation and those of the viable mutation and wild-type SRL may account for the impaired elongation factor binding to ribosomes with the C2658G*G2663C mutation and may underlie the lethal phenotype.
[[Category: Correll CC]]
 
[[Category: Lubbers M]]
==About this Structure==
[[Category: Plantinga MJ]]
1Q9A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q9A OCA].  
 
==Reference==
The common and the distinctive features of the bulged-G motif based on a 1.04 A resolution RNA structure., Correll CC, Beneken J, Plantinga MJ, Lubbers M, Chan YL, Nucleic Acids Res. 2003 Dec 1;31(23):6806-18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14627814 14627814]
[[Category: Single protein]]
[[Category: Beneken, J.]]
[[Category: Chan, Y L.]]
[[Category: Correll, C C.]]
[[Category: Lubbers, M.]]
[[Category: Plantinga, M J.]]
[[Category: high resolution]]
[[Category: ribonucleic acid]]
[[Category: ribosome]]
[[Category: rna recognition]]
[[Category: sarcin/ricin domain]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:34:43 2008''

Latest revision as of 11:14, 14 February 2024

Crystal structure of the sarcin/ricin domain from E.coli 23S rRNA at 1.04 resolutionCrystal structure of the sarcin/ricin domain from E.coli 23S rRNA at 1.04 resolution

Structural highlights

1q9a is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.04Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

1q9a, resolution 1.04Å

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