1q07: Difference between revisions

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-[[Image:1q07.gif|left|200px]]<br /><applet load="1q07" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1q07, resolution 2.50&Aring;" />
-'''Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator'''<br />
-==Overview==
+==Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator==
-The earliest of a series of copper efflux genes in Escherichia coli are controlled by CueR, a member of the MerR family of transcriptional activators. Thermodynamic calibration of CueR reveals a zeptomolar (10(-21) molar) sensitivity to free Cu+, which is far less than one atom per cell. Atomic details of this extraordinary sensitivity and selectivity for +1transition-metal ions are revealed by comparing the crystal structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding interactions to enhance metal binding. This binding mode is rare among metalloproteins but well suited for an ultrasensitive genetic switch.
+<StructureSection load='1q07' size='340' side='right'caption='[[1q07]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1q07]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q07 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q07 OCA], [https://pdbe.org/1q07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q07 RCSB], [https://www.ebi.ac.uk/pdbsum/1q07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q07 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CUER_ECOLI CUER_ECOLI] Regulates the transcription of the copA and cueO genes. It detects cytoplasmic copper stress and activates transcription in response to increasing copper concentrations.<ref>PMID:10915804</ref> <ref>PMID:11399769</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q0/1q07_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q07 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-Q07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=AU:'>AU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q07 OCA].
+*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
+== References ==
-==Reference==
+<references/>
-Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR., Changela A, Chen K, Xue Y, Holschen J, Outten CE, O'Halloran TV, Mondragon A, Science. 2003 Sep 5;301(5638):1383-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12958362 12958362]
+__TOC__
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Changela, A.]]
+[[Category: Changela A]]
-[[Category: Chen, K.]]
+[[Category: Chen K]]
-[[Category: Halloran, T V.O.]]
+[[Category: Holschen J]]
-[[Category: Holschen, J.]]
+[[Category: Mondragon A]]
-[[Category: Mondragon, A.]]
+[[Category: O'Halloran TV]]
-[[Category: Outten, C E.]]
+[[Category: Outten CE]]
-[[Category: Xue, Y.]]
+[[Category: Xue Y]]
-[[Category: AU]]
-[[Category: copper efflux regulator]]
-[[Category: merr family transcriptional regulator]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:26 2008''