1pxt: Difference between revisions

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New page: left|200px<br /><applet load="1pxt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pxt, resolution 2.8Å" /> '''THE 2.8 ANGSTROMS STR...
 
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[[Image:1pxt.jpg|left|200px]]<br /><applet load="1pxt" size="450" color="white" frame="true" align="right" spinBox="true"
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'''THE 2.8 ANGSTROMS STRUCTURE OF PEROXISOMAL 3-KETOACYL-COA THIOLASE OF SACCHAROMYCES CEREVISIAE: A FIVE LAYERED A-B-A-B-A STRUCTURE, CONSTRUCTED FROM TWO CORE DOMAINS OF IDENTICAL TOPOLOGY'''<br />


==Overview==
==THE 2.8 ANGSTROMS STRUCTURE OF PEROXISOMAL 3-KETOACYL-COA THIOLASE OF SACCHAROMYCES CEREVISIAE: A FIVE LAYERED A-B-A-B-A STRUCTURE, CONSTRUCTED FROM TWO CORE DOMAINS OF IDENTICAL TOPOLOGY==
BACKGROUND: The peroxisomal enzyme 3-ketoacyl-coenzyme A thiolase of the, yeast Saccharomyces cerevisiae is a homodimer with 417 residues per, subunit. It is synthesized in the cytosol and subsequently imported into, the peroxisome where it catalyzes the last step of the beta-oxidation, pathway. We have determined the structure of this thiolase in order to, study the reaction mechanism, quaternary associations and intracellular, targeting of thiolases generally, and to understand the structural basis, of genetic disorders associated with human thiolases. RESULTS: Here we, report the crystal structure of unliganded yeast thiolase refined at 2.8 A, resolution. The enzyme comprises three domains; two compact core domains, having the same fold and a loop domain. Each of the two core domains is, folded into a mixed five-stranded beta-sheet covered on each side by, helices and the two are assembled into a five-layered alpha beta alpha, beta alpha structure. The central layer is formed by two helices, which, point with their amino termini towards the active site. The loop domain, which is to some extent stabilized by interactions with the other subunit, runs over the surface of the two core domains, encircling the active site, of its own subunit. CONCLUSIONS: The crystal structure of thiolase shows, that the active site is a shallow pocket, shaped by highly conserved, residues. Two conserved cysteines and a histidine at the floor of this, pocket probably play key roles in the reaction mechanism. The two active, sites are on the same face of the dimer, far from the amino and carboxyl, termini of both subunits and the disordered amino-terminal import signal, sequence.
<StructureSection load='1pxt' size='340' side='right'caption='[[1pxt]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1pxt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PXT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pxt OCA], [https://pdbe.org/1pxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pxt RCSB], [https://www.ebi.ac.uk/pdbsum/1pxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pxt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/THIK_YEAST THIK_YEAST]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/px/1pxt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pxt ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1PXT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/Acetyl-CoA_C-acyltransferase Acetyl-CoA C-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.16 2.3.1.16] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PXT OCA].
*[[Thiolase 3D structures|Thiolase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The 2.8 A crystal structure of peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: a five-layered alpha beta alpha beta alpha structure constructed from two core domains of identical topology., Mathieu M, Zeelen JP, Pauptit RA, Erdmann R, Kunau WH, Wierenga RK, Structure. 1994 Sep 15;2(9):797-808. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7812714 7812714]
[[Category: Large Structures]]
[[Category: Acetyl-CoA C-acyltransferase]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Mathieu M]]
[[Category: Mathieu, M.]]
[[Category: Wierenga RK]]
[[Category: Wierenga, R.K.]]
[[Category: thiolase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:12:57 2007''

Latest revision as of 11:11, 14 February 2024

THE 2.8 ANGSTROMS STRUCTURE OF PEROXISOMAL 3-KETOACYL-COA THIOLASE OF SACCHAROMYCES CEREVISIAE: A FIVE LAYERED A-B-A-B-A STRUCTURE, CONSTRUCTED FROM TWO CORE DOMAINS OF IDENTICAL TOPOLOGYTHE 2.8 ANGSTROMS STRUCTURE OF PEROXISOMAL 3-KETOACYL-COA THIOLASE OF SACCHAROMYCES CEREVISIAE: A FIVE LAYERED A-B-A-B-A STRUCTURE, CONSTRUCTED FROM TWO CORE DOMAINS OF IDENTICAL TOPOLOGY

Structural highlights

1pxt is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

THIK_YEAST

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1pxt, resolution 2.80Å

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