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| [[Image:1p3h.gif|left|200px]]
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| {{Structure
| | ==Crystal Structure of the Mycobacterium tuberculosis chaperonin 10 tetradecamer== |
| |PDB= 1p3h |SIZE=350|CAPTION= <scene name='initialview01'>1p3h</scene>, resolution 2.8Å
| | <StructureSection load='1p3h' size='340' side='right'caption='[[1p3h]], [[Resolution|resolution]] 2.80Å' scene=''> |
| |SITE= | | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>
| | <table><tr><td colspan='2'>[[1p3h]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1jh2 1jh2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P3H FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| |GENE= GROS OR GROES OR MOPB OR CPN10 OR RV3418C OR MT3527 OR MTCY78.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> |
| |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00320 cpn10]</span>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p3h OCA], [https://pdbe.org/1p3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p3h RCSB], [https://www.ebi.ac.uk/pdbsum/1p3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p3h ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p3h OCA], [http://www.ebi.ac.uk/pdbsum/1p3h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p3h RCSB]</span>
| | == Function == |
| }}
| | [https://www.uniprot.org/uniprot/CH10_MYCTU CH10_MYCTU] Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.[HAMAP-Rule:MF_00580] |
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p3/1p3h_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p3h ConSurf]. |
| | <div style="clear:both"></div> |
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| '''Crystal Structure of the Mycobacterium tuberculosis chaperonin 10 tetradecamer'''
| | ==See Also== |
| | | *[[Chaperonin 3D structures|Chaperonin 3D structures]] |
| | | __TOC__ |
| ==Overview==
| | </StructureSection> |
| The crystal structure of Mycobacterium tuberculosis chaperonin 10 (cpn10(Mt)) has been determined to a resolution of 2.8 A. Two dome-shaped cpn10(Mt) heptamers complex through loops at their bases to form a tetradecamer with 72 symmetry and a spherical cage-like structure. The hollow interior enclosed by the tetradecamer is lined with hydrophilic residues and has dimensions of 30 A perpendicular to and 60 A along the sevenfold axis. Tetradecameric cpn10(Mt) has also been observed in solution by dynamic light scattering. Through its base loop sequence cpn10(Mt) is known to be the agent in the bacterium responsible for bone resorption and for the contribution towards its strong T-cell immunogenicity. Superimposition of the cpn10(Mt) sequences 26 to 32 and 66 to 72 and E. coli GroES 25 to 31 associated with bone resorption activity shows them to have similar conformations and structural features, suggesting that there may be a common receptor for the bone resorption sequences. The base loops of cpn10s in general also attach to the corresponding chaperonin 60 (cpn60) to enclose unfolded protein and to facilitate its correct folding in vivo. Electron density corresponding to a partially disordered protein subunit appears encapsulated within the interior dome cavity of each heptamer. This suggests that the binding of substrates to cpn10 is possible in the absence of cpn60.
| | [[Category: Large Structures]] |
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| ==About this Structure==
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| 1P3H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry 1JH2. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P3H OCA].
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| ==Reference==
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| Mycobacterium tuberculosis chaperonin 10 heptamers self-associate through their biologically active loops., Roberts MM, Coker AR, Fossati G, Mascagni P, Coates AR, Wood SP, J Bacteriol. 2003 Jul;185(14):4172-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12837792 12837792]
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| [[Category: Mycobacterium tuberculosis]] | | [[Category: Mycobacterium tuberculosis]] |
| [[Category: Single protein]]
| | [[Category: Coates ARM]] |
| [[Category: Coates, A R.M.]] | | [[Category: Coker AR]] |
| [[Category: Coker, A R.]] | | [[Category: Fossati G]] |
| [[Category: Fossati, G.]] | | [[Category: Mascagni P]] |
| [[Category: Mascagni, P.]] | | [[Category: Roberts MM]] |
| [[Category: Roberts, M M.]] | | [[Category: Wood SP]] |
| [[Category: TBSGC, TB Structural Genomics Consortium.]]
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| [[Category: Wood, S P.]] | |
| [[Category: acidic cluster]]
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| [[Category: beta barrel]]
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| [[Category: flexible loop]]
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| [[Category: protein structure initiative]]
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| [[Category: psi]]
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| [[Category: structural genomic]]
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| [[Category: tb structural genomics consortium]]
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| [[Category: tbsgc]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:54:20 2008''
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